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Literature summary for 3.2.1.41 extracted from

  • Bertoldo, C.; Armbrecht, M.; Becker, F.; Schafer, T.; Antranikian, G.; Liebl, W.
    Cloning, sequencing, and characterization of a heat- and alkali-stable type I pullulanase from Anaerobranca gottschalkii (2004), Appl. Environ. Microbiol., 70, 3407-3416.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.2.1.41

Cloned(Commentary)

Cloned (Comment) Organism
-
 Anaerobranca gottschalkii

Protein Variants

Protein Variants Comment Organism
additional information truncated form of enzyme, lacking first 252 amino acids, physicochemical properties almost identical to wild type enzyme Anaerobranca gottschalkii

Inhibitors

Inhibitors Comment Organism Structure
cyclodextrin
-
 Anaerobranca gottschalkii

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
96000
-
 x * 96000, mature protein, deduced from gene sequence Anaerobranca gottschalkii

Organism

Organism UniProt Comment Textmining
Anaerobranca gottschalkii Q6QHE9
-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
proteolytic modification protein contains a signal peptide Anaerobranca gottschalkii

Purification (Commentary)

Purification (Comment) Organism
expressed in Escherichia coli Anaerobranca gottschalkii

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3 glycogen + H2O
-
 Anaerobranca gottschalkii 4 maltotriose
-
 ?
amylopectin + H2O
-
 Anaerobranca gottschalkii maltotriose
-
 ?
additional information no substrate: amylose Anaerobranca gottschalkii ?
-
 ?
pullulan + H2O
-
 Anaerobranca gottschalkii maltotriose + ? final product ?
starch + H2O
-
 Anaerobranca gottschalkii maltotriose + ?
-
 ?

Subunits

Subunits Comment Organism
? x * 96000, mature protein, deduced from gene sequence Anaerobranca gottschalkii

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
70
-
-
 Anaerobranca gottschalkii

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
70
-
 22h, 50% loss of activity Anaerobranca gottschalkii

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
-
 Anaerobranca gottschalkii