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Literature summary for 3.2.1.41 extracted from
- Improved high thermal stability of pullulanase from a newly isolated thermophilic Bacillus sp. AN-7 (2006), Enzyme Microb. Technol., 39, 1399-1404.
No PubMed abstract available
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| dithiothreitol | - |
Bacillus sp. (in: Bacteria) |  |
Application
| Application | Comment | Organism |
|---|---|---|
| industry | in the starch processing industry for the production of sugar syrups | Bacillus sp. (in: Bacteria) |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| additional information | EDTA has no effect, phenylmethanesulfonyl fluoride and diisopropylflurophosphate have almost no effect | Bacillus sp. (in: Bacteria) | |
| N-bromosuccinimide | inhibitory at 10mM | Bacillus sp. (in: Bacteria) | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| extracellular | - |
Bacillus sp. (in: Bacteria) | - |
- |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Ca2+ | activating | Bacillus sp. (in: Bacteria) | |
| Cu2+ | partially inactivating | Bacillus sp. (in: Bacteria) | |
| Hg2+ | complete inactivating | Bacillus sp. (in: Bacteria) | |
| Mn2+ | activating | Bacillus sp. (in: Bacteria) | |
| additional information | Fe2+, Mg2+, Ba2+, K+, Na+ or Zn2+ have no effect | Bacillus sp. (in: Bacteria) |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 106000 | - |
SDS-PAGE | Bacillus sp. (in: Bacteria) |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Bacillus sp. (in: Bacteria) | - |
from soil in India | - |
| Bacillus sp. (in: Bacteria) AN-7 | - |
from soil in India | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| ammonium sulfate precipitation, anion exchange and gel filtration chromatography | Bacillus sp. (in: Bacteria) |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 151.5 | - |
after purification | Bacillus sp. (in: Bacteria) |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| pullulan + H2O | - |
Bacillus sp. (in: Bacteria) | maltotriose + maltose | - |
? | |
| pullulan + H2O | - |
Bacillus sp. (in: Bacteria) AN-7 | maltotriose + maltose | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| pullulanase | - |
Bacillus sp. (in: Bacteria) |
| pullulanase type I | - |
Bacillus sp. (in: Bacteria) |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 90 | - |
- |
Bacillus sp. (in: Bacteria) |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 80 | 100 | - |
Bacillus sp. (in: Bacteria) |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6 | - |
- |
Bacillus sp. (in: Bacteria) |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 5 | 7 | - |
Bacillus sp. (in: Bacteria) |
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