Cloned (Comment) | Organism |
---|---|
homologously expressed in Sulfolobus acidocaldarius | Sulfolobus acidocaldarius |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
SDS | 1%, complete inactivation | Sulfolobus acidocaldarius |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
membrane | - |
Sulfolobus acidocaldarius | 16020 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Co2+ | 1 mM CoCl2, slightly enhances activity | Sulfolobus acidocaldarius | |
Mg2+ | 1 mM MhCl2, slightly enhances activity | Sulfolobus acidocaldarius |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
100000 | - |
4 * 100000, SDS-PAGE | Sulfolobus acidocaldarius |
435000 | - |
gel filtration | Sulfolobus acidocaldarius |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Sulfolobus acidocaldarius | Q4J9M2 | - |
- |
Sulfolobus acidocaldarius DSM 639 | Q4J9M2 | - |
- |
Purification (Comment) | Organism |
---|---|
- |
Sulfolobus acidocaldarius |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
amylopectin + H2O | the enzyme can degrade both the alpha-1,4 and alpha-1,6-linkages of alpha-glucans | Sulfolobus acidocaldarius | D-glucose + maltose + maltotriose | - |
? | |
amylopectin + H2O | the enzyme can degrade both the alpha-1,4 and alpha-1,6-linkages of alpha-glucans | Sulfolobus acidocaldarius DSM 639 | D-glucose + maltose + maltotriose | - |
? | |
glycogen + H2O | the enzyme can degrade both alpha-1,4 and alpha-1,6-linkages of alpha-glucan | Sulfolobus acidocaldarius | ? | - |
? | |
glycogen + H2O | the enzyme can degrade both alpha-1,4 and alpha-1,6-linkages of alpha-glucan | Sulfolobus acidocaldarius DSM 639 | ? | - |
? | |
additional information | the enzyme can degrade both alpha-1,4 and alpha-1,6-linkages of alpha-glucan | Sulfolobus acidocaldarius | ? | - |
? | |
additional information | the enzyme can degrade both alpha-1,4 and alpha-1,6-linkages of alpha-glucan | Sulfolobus acidocaldarius DSM 639 | ? | - |
? | |
starch + H2O | the enzyme can degrade both the alpha-1,4 and alpha-1,6-linkages of alpha-glucans | Sulfolobus acidocaldarius | D-glucose + maltose + maltotriose | - |
? | |
starch + H2O | the enzyme can degrade both the alpha-1,4 and alpha-1,6-linkages of alpha-glucans | Sulfolobus acidocaldarius DSM 639 | D-glucose + maltose + maltotriose | - |
? |
Subunits | Comment | Organism |
---|---|---|
tetramer | 4 * 100000, SDS-PAGE | Sulfolobus acidocaldarius |
Synonyms | Comment | Organism |
---|---|---|
amylopullulanase | - |
Sulfolobus acidocaldarius |
Apu | - |
Sulfolobus acidocaldarius |
Saci_1162 | locus name | Sulfolobus acidocaldarius |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
95 | 105 | amylolytic activity toward starch | Sulfolobus acidocaldarius |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
80 | 110 | 80°C: about 55% of maximal activity, 110°C: about 50% of maximal activity, amylolytic activity toward starch | Sulfolobus acidocaldarius |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
90 | 95 | 40 h, stable | Sulfolobus acidocaldarius |
100 | - |
half-life: 2.5 h | Sulfolobus acidocaldarius |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
3 | 3.5 | amylolytic activity towards starch | Sulfolobus acidocaldarius |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
2 | 5 | pH 2.0: about 60% of maximal activity, pH 5.0: about 60% of maximal activity, , amylolytic activity toward starch | Sulfolobus acidocaldarius |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Sulfolobus acidocaldarius | calculated from sequence | - |
5.3 |
General Information | Comment | Organism |
---|---|---|
metabolism | the enzyme is essential for starch metabolism | Sulfolobus acidocaldarius |