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Literature summary for 3.2.1.41 extracted from

  • Choi KH, Cha J.
    Membrane-bound amylopullulanase is essential for starch metabolism of Sulfolobus acidocaldarius DSM639 (2015), Extremophiles, 19, 909-920.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
homologously expressed in Sulfolobus acidocaldarius Sulfolobus acidocaldarius

Inhibitors

Inhibitors Comment Organism Structure
SDS 1%, complete inactivation Sulfolobus acidocaldarius

Localization

Localization Comment Organism GeneOntology No. Textmining
membrane
-
Sulfolobus acidocaldarius 16020
-

Metals/Ions

Metals/Ions Comment Organism Structure
Co2+ 1 mM CoCl2, slightly enhances activity Sulfolobus acidocaldarius
Mg2+ 1 mM MhCl2, slightly enhances activity Sulfolobus acidocaldarius

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
100000
-
4 * 100000, SDS-PAGE Sulfolobus acidocaldarius
435000
-
gel filtration Sulfolobus acidocaldarius

Organism

Organism UniProt Comment Textmining
Sulfolobus acidocaldarius Q4J9M2
-
-
Sulfolobus acidocaldarius DSM 639 Q4J9M2
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Sulfolobus acidocaldarius

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
amylopectin + H2O the enzyme can degrade both the alpha-1,4 and alpha-1,6-linkages of alpha-glucans Sulfolobus acidocaldarius D-glucose + maltose + maltotriose
-
?
amylopectin + H2O the enzyme can degrade both the alpha-1,4 and alpha-1,6-linkages of alpha-glucans Sulfolobus acidocaldarius DSM 639 D-glucose + maltose + maltotriose
-
?
glycogen + H2O the enzyme can degrade both alpha-1,4 and alpha-1,6-linkages of alpha-glucan Sulfolobus acidocaldarius ?
-
?
glycogen + H2O the enzyme can degrade both alpha-1,4 and alpha-1,6-linkages of alpha-glucan Sulfolobus acidocaldarius DSM 639 ?
-
?
additional information the enzyme can degrade both alpha-1,4 and alpha-1,6-linkages of alpha-glucan Sulfolobus acidocaldarius ?
-
?
additional information the enzyme can degrade both alpha-1,4 and alpha-1,6-linkages of alpha-glucan Sulfolobus acidocaldarius DSM 639 ?
-
?
starch + H2O the enzyme can degrade both the alpha-1,4 and alpha-1,6-linkages of alpha-glucans Sulfolobus acidocaldarius D-glucose + maltose + maltotriose
-
?
starch + H2O the enzyme can degrade both the alpha-1,4 and alpha-1,6-linkages of alpha-glucans Sulfolobus acidocaldarius DSM 639 D-glucose + maltose + maltotriose
-
?

Subunits

Subunits Comment Organism
tetramer 4 * 100000, SDS-PAGE Sulfolobus acidocaldarius

Synonyms

Synonyms Comment Organism
amylopullulanase
-
Sulfolobus acidocaldarius
Apu
-
Sulfolobus acidocaldarius
Saci_1162 locus name Sulfolobus acidocaldarius

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
95 105 amylolytic activity toward starch Sulfolobus acidocaldarius

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
80 110 80°C: about 55% of maximal activity, 110°C: about 50% of maximal activity, amylolytic activity toward starch Sulfolobus acidocaldarius

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
90 95 40 h, stable Sulfolobus acidocaldarius
100
-
half-life: 2.5 h Sulfolobus acidocaldarius

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
3 3.5 amylolytic activity towards starch Sulfolobus acidocaldarius

pH Range

pH Minimum pH Maximum Comment Organism
2 5 pH 2.0: about 60% of maximal activity, pH 5.0: about 60% of maximal activity, , amylolytic activity toward starch Sulfolobus acidocaldarius

pI Value

Organism Comment pI Value Maximum pI Value
Sulfolobus acidocaldarius calculated from sequence
-
5.3

General Information

General Information Comment Organism
metabolism the enzyme is essential for starch metabolism Sulfolobus acidocaldarius