Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 3.2.1.41 extracted from

  • Rajaei, S.; Noghabi, K.A.; Sadeghizadeh, M.; Zahiri, H.S.
    Characterization of a pH and detergent-tolerant, cold-adapted type I pullulanase from Exiguobacterium sp. SH3 (2015), Extremophiles, 19, 1145-1155.
    View publication on PubMed
Search for more literature for 3.2.1.41

Activating Compound

Activating Compound Comment Organism Structure
2-mercaptoethanol 1% v/v, 149% of initial activity Exiguobacterium sp.
dithiothreitol 0.2% w/v, 147% of initial activity Exiguobacterium sp.

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli and Bacillus subtilis Exiguobacterium sp.

Inhibitors

Inhibitors Comment Organism Structure
Cu2+ 2 mM, 79% residual activity Exiguobacterium sp.
additional information enzyme retains 99, 89, and 54% of its activity at 10% concentration of Triton-X100, Tween 20, and SDS, respectively. The enzyme retains 80.4 and 93.7% activity in the presence of commercial detergents Rika (7.5% v/v) and Fadisheh (2.5% w/v), respectively Exiguobacterium sp.
Zn2+ 2 mM, 48% residual activity Exiguobacterium sp.

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
 pullulan Km value is 2.8 mg/ml, pH 8.5, 45°C Exiguobacterium sp.

Metals/Ions

Metals/Ions Comment Organism Structure
Co2+ 2 mM, 113% of initial activity Exiguobacterium sp.
Mn2+ 2 mM, 143% of initial activity Exiguobacterium sp.

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
110000
-
 x * 110000, SDS-PAGE Exiguobacterium sp.

Organism

Organism UniProt Comment Textmining
Exiguobacterium sp. A0A0B5JT51
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information negligible activity on wheat flour and maltodextrin, no activity on amylose Exiguobacterium sp. ?
-
 ?
potato starch + H2O 14% of the activity with pullulan Exiguobacterium sp. ?
-
 ?
pullulan + H2O
-
 Exiguobacterium sp. ?
-
 ?
rice flour + H2O 15% of the activity with pullulan Exiguobacterium sp. ?
-
 ?
soluble starch + H2O 17% of the activity with pullulan Exiguobacterium sp. ?
-
 ?

Subunits

Subunits Comment Organism
? x * 110000, SDS-PAGE Exiguobacterium sp.

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
45
-
-
 Exiguobacterium sp.

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
52
-
 melting temperature Exiguobacterium sp.

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
37
-
 pullulan pH 8.5, 45°C Exiguobacterium sp.

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8.5
-
-
 Exiguobacterium sp.

pH Range

pH Minimum pH Maximum Comment Organism
5 11 more than 60% of maximum activity Exiguobacterium sp.