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Literature summary for 3.2.1.41 extracted from

  • Shehata, A.; Darwish, D.; Masoud, H.
    Extraction, purification and characterization of endo-acting pullulanase type I from white edible mushrooms (2016), J. Appl. Pharm. Sci., 6, 147-152 .
No PubMed abstract available
Search for more literature for 3.2.1.41

Activating Compound

Activating Compound Comment Organism Structure
dithiothreitol 1 M, 1.1fold activation unidentified
EDTA 1 M, 1.3fold activation unidentified

Inhibitors

Inhibitors Comment Organism Structure
Ag+ 0.1 M, 50% loss of activity unidentified
Hg2+ 0.1 M, 70% loss of activity unidentified
iodoacetate 1 M, 10% loss of activity unidentified
Sodium fluoride 1 M, 10% loss of activity unidentified

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.27
-
 pullulan pH 6, 40°C unidentified

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ 0.1 M, 1.4fold activation unidentified
Fe2+ 0.1 M, 1.6fold activation unidentified
Mn2+ 0.1 M, 1.5fold activation unidentified

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
112000
-
 SDS-PAGE unidentified

Organism

Organism UniProt Comment Textmining
unidentified
-
 edible white mushrooms
-

Purification (Commentary)

Purification (Comment) Organism
-
 unidentified

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
amylopectin + H2O hydrolyzed at 60% compared to pullulan unidentified ?
-
 ?
glycogen + H2O hydrolyzed at 30% compared to pullulan unidentified ?
-
 ?
pullulan + H2O
-
 unidentified maltotriose + ?
-
 ?
soluble starch + H2O hydrolyzed at 80% compared to pullulan unidentified ?
-
 ?

Subunits

Subunits Comment Organism
? x * 112000, SDS-PAGE unidentified

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
40
-
-
 unidentified

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
30 70 30°C: about 60% of maximal activity, 70°C: about 40% of maximal activity unidentified

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
60
-
 30 min, 50% loss of activity unidentified
70
-
 45 min, 80% loss of activity unidentified

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6
-
-
 unidentified

pH Range

pH Minimum pH Maximum Comment Organism
5 8 pH 5.0: about 30% of maximal activity, pH 8.0: about 30% of maximal activity unidentified

pI Value

Organism Comment pI Value Maximum pI Value
unidentified isoelectric focusing
-
 6.2