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Literature summary for 3.2.1.52 extracted from

  • DeGaspari, R.; Li, S.C.; Li, Y.T.
    A GM2-specific beta-hexosaminidase from the roe of striped mullet (Mugil cephalus) (1988), J. Biol. Chem., 263, 1325-1328.
    View publication on PubMed
Search for more literature for 3.2.1.52

Activating Compound

Activating Compound Comment Organism Structure
low molecular weight protein restores the enzyme's ability to hydrolyze GM2 in high ionic strength Mugil cephalus

General Stability

General Stability Organism
high purification results in great loss of activity Mugil cephalus

Inhibitors

Inhibitors Comment Organism Structure
high ionic strength above 0.1 no cleavage of GM2 Mugil cephalus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
170000
-
 beta-hexosaminidase II + III Mugil cephalus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
N-acetyl-beta-D-hexosaminides + H2O Mugil cephalus
-
 N-acetyl-D-hexosamines
-
 ?

Organism

Organism UniProt Comment Textmining
Mugil cephalus
-
 striped mullet
-

Purification (Commentary)

Purification (Comment) Organism
beta-hexosaminidase II: 202fold , beta-hexosaminidase III: 426fold, still very heterogenous Mugil cephalus

Source Tissue

Source Tissue Comment Organism Textmining
roe
-
 Mugil cephalus
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
21.3
-
-
 Mugil cephalus

Storage Stability

Storage Stability Organism
-20°C, in 10 mM sodium acetate buffer pH 4.0, very unstable Mugil cephalus
-20°C, in 5-50 mM sodium citrate buffer pH 4.0, very stable, even after repeated freeze-thawing Mugil cephalus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
GM2 ganglioside + H2O
-
 Mugil cephalus GM3 ganglioside + N-acetyl-beta-D-galactosamine
-
 ?
N-acetyl-beta-D-hexosaminides + H2O
-
 Mugil cephalus N-acetyl-D-hexosamines
-
 ?
p-nitrophenyl-beta-2-acetamido-2-deoxy-D-galactopyranoside + H2O
-
 Mugil cephalus 2-acetamido-2-deoxy-D-galactose + p-nitrophenol
-
 ?
p-nitrophenyl-beta-2-acetamido-2-deoxy-D-glucopyranoside + H2O
-
 Mugil cephalus 2-acetamido-2-deoxy-D-glucose + p-nitrophenol
-
 ?
Sda oligosaccharide + H2O
-
 Mugil cephalus N-acetyl-D-galactosamine
-
 ?
SRO oligosaccharide + H2O
-
 Mugil cephalus N-acetyl-D-galactosamine
-
 ?

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
4
-
 substrate: p-nitrophenyl-beta-2-acetamido-2-deoxy-D-glucopyranoside Mugil cephalus