Activating Compound | Comment | Organism | Structure |
---|---|---|---|
2-mercaptoethanol | blocks inhibition by Hg2+-ions | Pisum sativum | |
Ca2+ | beta-NAHA-II | Pisum sativum | |
cysteine | prevents inhibition by Hg2+-ions | Pisum sativum | |
Mg2+ | beta-NAHA-II | Pisum sativum | |
Mn2+ | beta-NAHA-II | Pisum sativum |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Cu2+ | all 4 isozymes | Pisum sativum | |
Fe2+ | 2 mM causes 30-35% loss of activity of beta-NAHA-IA, 10 mM causes 30-35% loss of activity of beta-NAHA-IB | Pisum sativum | |
Hg2+ | all 4 isozymes, at 1 mM | Pisum sativum | |
p-nitrophenyl-beta-2-acetamido-2-deoxy-D-glucopyranoside | only beta-NAHA-IA, above 3.5 mM | Pisum sativum | |
Zn2+ | all 4 isozymes, at 10 mM inhibition ranges from 20% for beta-NAHA-IC to 45% for beta-NAHA-II | Pisum sativum |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.27 | - |
p-nitrophenyl-beta-2-acetamido-2-deoxy-D-galactopyranoside | beta-NAHA-IA | Pisum sativum | |
0.34 | - |
p-nitrophenyl-beta-2-acetamido-2-deoxy-D-galactopyranoside | beta-NAHA-IB | Pisum sativum | |
0.49 | - |
p-nitrophenyl-beta-2-acetamido-2-deoxy-D-glucopyranoside | beta-NAHA-IA | Pisum sativum | |
0.51 | - |
p-nitrophenyl-beta-2-acetamido-2-deoxy-D-glucopyranoside | beta-NAHA-IB | Pisum sativum | |
0.58 | - |
p-nitrophenyl-beta-2-acetamido-2-deoxy-D-galactopyranoside | beta-NAHA-II | Pisum sativum | |
0.61 | - |
p-nitrophenyl-beta-2-acetamido-2-deoxy-D-glucopyranoside | beta-NAHA-II | Pisum sativum | |
0.76 | - |
p-nitrophenyl-beta-2-acetamido-2-deoxy-D-glucopyranoside | beta-NAHA-IC | Pisum sativum | |
1.71 | - |
p-nitrophenyl-beta-2-acetamido-2-deoxy-D-galactopyranoside | beta-NAHA-IC | Pisum sativum |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
protein body | - |
Pisum sativum | 42735 | - |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
62000 | - |
beta-NAHA-II, 1 * 62000 + 1 * 64000 | Pisum sativum |
64000 | - |
beta-NAHA-II, 1 * 62000 + 1 * 64000 | Pisum sativum |
210000 | - |
gel filtration, all 4 isozymes | Pisum sativum |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
N-acetyl-beta-D-hexosaminides + H2O | Pisum sativum | - |
N-acetyl-D-hexosamines | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pisum sativum | - |
pea | - |
Posttranslational Modification | Comment | Organism |
---|---|---|
glycoprotein | - |
Pisum sativum |
Purification (Comment) | Organism |
---|---|
4 isozymes, beta-NAHA-II was purified 3200fold to apparent homogeneity, beta-NAHA-IA 770fold, beta-NAHA-IB 1750fold, beta-NAHA-IC 500fold | Pisum sativum |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
cotyledon | - |
Pisum sativum | - |
seed | - |
Pisum sativum | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
2.08 | - |
beta-NAHA-IC, substrate: p-nitrophenyl-beta-2-acetamido-2-deoxy-D-glucopyranoside | Pisum sativum |
3.17 | - |
beta-NAHA-IA, substrate: p-nitrophenyl-beta-2-acetamido-2-deoxy-D-glucopyranoside | Pisum sativum |
7.25 | - |
beta-NAHA-IB, substrate: p-nitrophenyl-beta-2-acetamido-2-deoxy-D-glucopyranoside | Pisum sativum |
13.4 | - |
beta-NAHA-II, substrate: p-nitrophenyl-beta-2-acetamido-2-deoxy-D-glucopyranoside | Pisum sativum |
Storage Stability | Organism |
---|---|
-20°C, lyophilized | Pisum sativum |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
N-acetyl-beta-D-hexosaminides + H2O | - |
Pisum sativum | N-acetyl-D-hexosamines | - |
? | |
p-nitrophenyl-beta-2-acetamido-2-deoxy-D-galactopyranoside + H2O | - |
Pisum sativum | 2-acetamido-2-deoxy-D-galactose + p-nitrophenol | - |
? | |
p-nitrophenyl-beta-2-acetamido-2-deoxy-D-glucopyranoside + H2O | - |
Pisum sativum | 2-acetamido-2-deoxy-D-glucose + p-nitrophenol | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | beta-NAHA-II, 1 * 62000 + 1 * 64000 | Pisum sativum |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
4.5 | 5 | all 4 isozymes, substrates: p-nitrophenyl-beta-2-acetamido-2-deoxy-D-glucopyranoside and p-nitrophenyl-beta-2-acetamido-2-deoxy-D-galactopyranoside | Pisum sativum |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
6 | - |
beta-NAHA-II, no hydrolysis at pH 6.0 and above, beta-NAHA-IA, -IB and IC show 20-25% of maximum activity at pH 6.0 | Pisum sativum |
6 | - |
beta-NAHA-II, no hydrolysis above at pH 6.0 and above, beta-NAHA-IA, -IB and IC show 20-25% of maximum activity at pH 6.0 | Pisum sativum |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
7 | - |
loss of 25% of acivity if pH is raised to 8.0 | Pisum sativum |