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Literature summary for 3.2.1.52 extracted from

  • Poulton, J.E.; Thomas, M.A.; Ottwell, K.K.; McCormick, S.J.
    Partial purification and characterization of a beta-N-acetylhexosaminidase from black cherry (Prunus serotina Ehrh.) seeds (1985), Plant Sci., 42, 107-114.
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General Stability

General Stability Organism
purification by N-acetylglucosamine-agarose columns results in extreme unstability of enzyme, extensive purification requires bovine serum albumin for retention of activity Prunus serotina

Inhibitors

Inhibitors Comment Organism Structure
Fe2+ 1 mM reduces activity by 59% Prunus serotina
Fe3+ concentration of 10 mM reduces activity by 23% Prunus serotina
Hg2+ 1 mM reduces activity by 89% Prunus serotina
N-acetyl-D-galactosamine 45% inhibition at 20 mM Prunus serotina
p-chloromercuriphenylsulfonate concentration of 10 mM inhibited activity by 46% Prunus serotina

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.49
-
 p-nitrophenyl-beta-2-acetamido-2-deoxy-D-glucopyranoside
-
 Prunus serotina
0.63
-
 4-nitrophenyl N-acetyl-beta-D-galactosaminide
-
 Prunus serotina

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
N-acetyl-beta-D-hexosaminides + H2O Prunus serotina
-
 N-acetyl-D-hexosamines
-
 ?

Organism

Organism UniProt Comment Textmining
Prunus serotina
-
 black cherry
-

Purification (Commentary)

Purification (Comment) Organism
partial purification, 30fold, more extensive purification requires bovine serum albumin for retention of activity Prunus serotina

Reaction

Reaction Comment Organism Reaction ID
(N-acetyl-D-glucosamine)4 + 3 H2O = 4 2-(acetylamino)-2-deoxy-beta-D-glucopyranose same active site for N-glucosaminidase and N-galactosaminidase activities Prunus serotina
a

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
184.2
-
 substrate: p-nitrophenyl-beta-2-acetamido-2-deoxy-D-glucopyranoside Prunus serotina

Storage Stability

Storage Stability Organism
4°C, enzyme purified in presence of bovine serum albumin, storage in presence of 0.04% w/v sodium azide, enzyme retains 76% of activity after 5 months Prunus serotina

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-nitrophenyl beta-D-galactopyranoside + H2O at 3.6% of the rate observed with p-nitrophenyl-beta-2-acetamido-2-deoxy-D-glucopyranoside Prunus serotina 4-nitrophenol + beta-D-galactopyranose
-
 ?
4-nitrophenyl N-acetyl-beta-D-galactosaminide + H2O
-
 Prunus serotina 4-nitrophenol + N-acetyl-beta-D-galactosamine
-
 ?
N-acetyl-beta-D-hexosaminides + H2O
-
 Prunus serotina N-acetyl-D-hexosamines
-
 ?
o-nitrophenyl-beta-D-glucoside + H2O at 4.2% of the rate observed with p-nitrophenyl-beta-2-acetamido-2-deoxy-D-glucopyranoside Prunus serotina 2-nitrophenol + beta-D-glucose
-
 ?
p-nitrophenyl-beta-2-acetamido-2-deoxy-D-galactopyranoside + H2O
-
 Prunus serotina 2-acetamido-2-deoxy-D-galactose + p-nitrophenol
-
 ?
p-nitrophenyl-beta-2-acetamido-2-deoxy-D-glucopyranoside + H2O
-
 Prunus serotina 2-acetamido-2-deoxy-D-glucose + p-nitrophenol
-
 ?
p-nitrophenyl-beta-D-glucopyranoside + H2O at 17% of the rate observed with p-nitrophenyl-beta-2-acetamido-2-deoxy-D-glucopyranoside Prunus serotina 4-nitrophenol + beta-D-glucose
-
 ?

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
4
-
 substrate: p-nitrophenyl-beta-2-acetamido-2-deoxy-D-galactopyranoside Prunus serotina
4.5
-
 substrate: p-nitrophenyl-beta-2-acetamido-2-deoxy-D-glucopyranoside Prunus serotina