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Literature summary for 3.2.1.52 extracted from
- Isolation of beta-N-acetylhexosaminidase from rabbit semen and its role in fertilization (1980), Biochem. J., 191, 827-834.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| glutathione | 1 mM leads to moderate stimulation of p-chloromercuribenzoate-treated enzyme, no effect alone | Oryctolagus cuniculus |  |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Ag+ | 1 and 2 mM cause 70 and 92% inhibition respectively | Oryctolagus cuniculus | |
| Hg2+ | 0.05 and 0.1 mM cause 45 and 70% inhibition | Oryctolagus cuniculus | |
| p-chloromercuribenzoate | 0.025 and 0.05 mM cause 70 and 83% inhibition | Oryctolagus cuniculus | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 1.4 | - |
p-nitrophenyl-beta-2-acetamido-2-deoxy-D-glucopyranoside | - |
Oryctolagus cuniculus | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| cytoplasm | - |
Oryctolagus cuniculus | 5737 | - |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| N-acetyl-beta-D-hexosaminides + H2O | Oryctolagus cuniculus | - |
N-acetyl-D-hexosamines | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Oryctolagus cuniculus | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| 226fold to homogeneity | Oryctolagus cuniculus |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| semen | - |
Oryctolagus cuniculus | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 56 | - |
substrate: p-nitrophenyl-beta-2-acetamido-2-deoxy-D-glucopyranoside | Oryctolagus cuniculus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| N-acetyl-beta-D-hexosaminides + H2O | - |
Oryctolagus cuniculus | N-acetyl-D-hexosamines | - |
? | |
| p-nitrophenyl-beta-2-acetamido-2-deoxy-D-glucopyranoside + H2O | - |
Oryctolagus cuniculus | 2-acetamido-2-deoxy-D-glucose + p-nitrophenol | - |
? | |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 55 | - |
purified enzyme loses 85% of activity after 20 min | Oryctolagus cuniculus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 4 | - |
substrate: p-nitrophenyl-beta-2-acetamido-2-deoxy-D-glucopyranoside | Oryctolagus cuniculus |
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