Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 3.2.1.52 extracted from

  • Verpoorte, J.A.
    Isolation and characterization of the major beta-N-acetyl-D-glucosaminidase from human plasma (1974), Biochemistry, 13, 793-799.
    View publication on PubMed
Search for more literature for 3.2.1.52

Activating Compound

Activating Compound Comment Organism Structure
bovine serum albumin
-
 Homo sapiens
cysteine prevents inhibition by Hg2+-ions Homo sapiens
cysteine prevents inhibition by Ag2+-ions Homo sapiens
Human serum albumin
-
 Homo sapiens

Inhibitors

Inhibitors Comment Organism Structure
acetate with 0.02 M 49% of activity remains Homo sapiens
Ag+ enzyme incubated before addition of substrate: with 0.0025 mM 18% of activity remains Homo sapiens
ascorbate only in presence of Cu2+ Homo sapiens
Cu2+ only in presence of ascorbate Homo sapiens
Fe2+ with 0.2 mM 28% of activity remains, citrate buffer, not inhibitory in succinate or acetate buffer Homo sapiens
Hg2+ added with substrate: with 0.0025 mM 45% of activity remains, enzyme incubated before addition of substrate: with 0.0025 mM 25% of activity remains Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.108
-
 4-methylumbelliferyl-2-acetamido-2-deoxy-beta-D-galactopyranoside
-
 Homo sapiens
0.17
-
 p-nitrophenyl-beta-2-acetamido-2-deoxy-D-galactopyranoside
-
 Homo sapiens
0.555
-
 4-methylumbelliferyl-2-acetamido-2-deoxy-beta-D-glucopyranoside with 0.01% bovine or human serum albumin Homo sapiens
0.58
-
 4-methylumbelliferyl-2-acetamido-2-deoxy-beta-D-glucopyranoside
-
 Homo sapiens
0.83
-
 p-nitrophenyl-beta-2-acetamido-2-deoxy-D-glucopyranoside
-
 Homo sapiens

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
105000
-
 sedimentation equilibrium using untreated enzyme, carboxymethylated enzyme in 6 M guanidine-HCl, meniscus depletion experiments with fresh enzyme prepatation at pH 11.5 Homo sapiens
107000
-
 gel filtration Homo sapiens
120000
-
 enzyme after dialysis and freeze-drying, 6 M guanidine-HCl, pH 7.2 Homo sapiens
260000
-
 pH 2.5, fresh enzyme prepatation, meniscus depletion experiments Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
N-acetyl-beta-D-hexosaminides + H2O Homo sapiens
-
 N-acetyl-D-hexosamines
-
 ?

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
glycoprotein glucosaminylation Homo sapiens
glycoprotein sialylation Homo sapiens
glycoprotein galactosylation Homo sapiens

Purification (Commentary)

Purification (Comment) Organism
195fold to homogeneity Homo sapiens

Source Tissue

Source Tissue Comment Organism Textmining
serum
-
 Homo sapiens
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-methylumbelliferyl-2-acetamido-2-deoxy-beta-D-galactopyranoside + H2O
-
 Homo sapiens 4-methylumbelliferone + 2-acetamido-2-deoxy-beta-D-galactopyranoside
-
 ?
4-methylumbelliferyl-2-acetamido-2-deoxy-beta-D-glucopyranoside + H2O
-
 Homo sapiens 4-methylumbelliferone + 2-acetamido-2-deoxy-beta-D-glucopyranoside
-
 ?
N-acetyl-beta-D-hexosaminides + H2O
-
 Homo sapiens N-acetyl-D-hexosamines
-
 ?
p-nitrophenyl-beta-2-acetamido-2-deoxy-D-galactopyranoside + H2O
-
 Homo sapiens 2-acetamido-2-deoxy-D-galactose + p-nitrophenol
-
 ?
p-nitrophenyl-beta-2-acetamido-2-deoxy-D-glucopyranoside + H2O
-
 Homo sapiens 2-acetamido-2-deoxy-D-glucose + p-nitrophenol
-
 ?

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
4.5 5
-
 Homo sapiens