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Literature summary for 3.2.1.54 extracted from
- Purification and characterization of a cyclodextrin-degrading enzyme from Flavobacterium sp. (1993), Appl. Microbiol. Biotechnol., 39, 714-719.
No PubMed abstract available
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.244 | - |
beta-cyclodextrin | - |
Flavobacterium sp. |  |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| cell bound | - |
Flavobacterium sp. | - |
- |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 62000 | - |
- |
Flavobacterium sp. |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Flavobacterium sp. | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| purified to 94% homogeneity | Flavobacterium sp. |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 60 | - |
CM-Servacel, pH 6.7 | Flavobacterium sp. |
| 100 | - |
beta-cyclodextrin as substrate | Flavobacterium sp. |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| alpha-cyclodextrin + H2O | - |
Flavobacterium sp. | glucose + maltose + maltotriose | - |
? | |
| cyclomaltodextrin + H2O | - |
Flavobacterium sp. | maltodextrin | - |
? | |
| gamma-cyclodextrin + H2O | - |
Flavobacterium sp. | glucose + maltose + maltotriose + maltotetraose + maltopentaose + maltohexaose + maltoheptaose + maltooctaose | - |
? | |
| maltodextrins + H2O | 6 to 8 membered maltooligosaccharides | Flavobacterium sp. | maltose + glucose | - |
? | |
| maltoheptaose + H2O | - |
Flavobacterium sp. | D-glucose + maltose + ? | - |
? | |
| maltohexaose + H2O | - |
Flavobacterium sp. | maltotetraose + maltose | - |
? | |
| maltotriose + H2O | - |
Flavobacterium sp. | ? | - |
? | |
| pullulan + H2O | - |
Flavobacterium sp. | glucose + panose + isopanose + large saccharides containing alpha 1,6-glucosidic bonds | - |
? | |
| starch + H2O | - |
Flavobacterium sp. | glucose + maltose | glucose minor early product, + maltotriose + maltotetraose | ? | |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 60 | - |
enzyme is not thermostable, above 60°C more than 80% activity loss | Flavobacterium sp. |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6 | 7.5 | - |
Flavobacterium sp. |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 5.8 | 7.8 | - |
Flavobacterium sp. |
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