Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21(DE3) cells | Palaeococcus pacificus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | 82.87% residual activity at 5 mM | Palaeococcus pacificus | |
Fe3+ | complete inhibition at 5 mM | Palaeococcus pacificus | |
Mg2+ | 99.6% residual activity at 5 mM | Palaeococcus pacificus | |
Zn2+ | complete inhibition at 5 mM | Palaeococcus pacificus |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
K+ | 102.7% activity at 5 mM | Palaeococcus pacificus | |
Li+ | 109% activity at 5 mM | Palaeococcus pacificus | |
Na+ | 103% activity at 5 mM | Palaeococcus pacificus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Palaeococcus pacificus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
Ni-NTA column chromatography, and Superdex 200 gel filtration | Palaeococcus pacificus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
alpha-cyclodextrin + H2O | highest activity. The enzyme shows strongest preference towards alpha-and beta-cyclodextrin | Palaeococcus pacificus | maltohexaose | - |
? | |
beta-cyclodextrin + H2O | strongest preference towards alpha-and beta-cyclodextrin | Palaeococcus pacificus | maltoheptaose | - |
? | |
gamma-cyclodextrin + H2O | - |
Palaeococcus pacificus | maltooctaose | - |
? | |
maltoheptaose + H2O | - |
Palaeococcus pacificus | maltotetraose + maltotriose | - |
? | |
maltotetraose + H2O | - |
Palaeococcus pacificus | 2 maltose | - |
? | |
maltotriose + H2O | - |
Palaeococcus pacificus | maltose + D-glucose | - |
? | |
additional information | the enzyme hardly reacts with potato soluble starch, pullulan, amylose, amylopectin | Palaeococcus pacificus | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
cyclodextrinase | - |
Palaeococcus pacificus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
95 | - |
- |
Palaeococcus pacificus |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
70 | 100 | more than 50% activity between 70 and 100°C | Palaeococcus pacificus |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
75 | 95 | the enzyme retains more than 90% activity after 8 h at 75-85°C. At 95°C, the enzyme shows about 77%, 45%, 30% and less than 20% activity after 1 h, 2 h, 4 h and 8 h, respectively | Palaeococcus pacificus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6 | - |
- |
Palaeococcus pacificus |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
5 | 8 | about 87% activity at pH 5.0, 100% activity at pH 6.0, about 75% activity at pH 7.0, about 50% activity at pH 8.0 | Palaeococcus pacificus |