Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 3.2.1.54 extracted from

  • Ji, H.; Bai, Y.; Li, X.; Wang, J.; Xu, X.; Jin, Z.
    Preparation of malto-oligosaccharides with specific degree of polymerization by a novel cyclodextrinase from Palaeococcus pacificus (2019), Carbohydr. Polym., 210, 64-72 .
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3) cells Palaeococcus pacificus

Inhibitors

Inhibitors Comment Organism Structure
Ca2+ 82.87% residual activity at 5 mM Palaeococcus pacificus
Fe3+ complete inhibition at 5 mM Palaeococcus pacificus
Mg2+ 99.6% residual activity at 5 mM Palaeococcus pacificus
Zn2+ complete inhibition at 5 mM Palaeococcus pacificus

Metals/Ions

Metals/Ions Comment Organism Structure
K+ 102.7% activity at 5 mM Palaeococcus pacificus
Li+ 109% activity at 5 mM Palaeococcus pacificus
Na+ 103% activity at 5 mM Palaeococcus pacificus

Organism

Organism UniProt Comment Textmining
Palaeococcus pacificus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
Ni-NTA column chromatography, and Superdex 200 gel filtration Palaeococcus pacificus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
alpha-cyclodextrin + H2O highest activity. The enzyme shows strongest preference towards alpha-and beta-cyclodextrin Palaeococcus pacificus maltohexaose
-
?
beta-cyclodextrin + H2O strongest preference towards alpha-and beta-cyclodextrin Palaeococcus pacificus maltoheptaose
-
?
gamma-cyclodextrin + H2O
-
Palaeococcus pacificus maltooctaose
-
?
maltoheptaose + H2O
-
Palaeococcus pacificus maltotetraose + maltotriose
-
?
maltotetraose + H2O
-
Palaeococcus pacificus 2 maltose
-
?
maltotriose + H2O
-
Palaeococcus pacificus maltose + D-glucose
-
?
additional information the enzyme hardly reacts with potato soluble starch, pullulan, amylose, amylopectin Palaeococcus pacificus ?
-
?

Synonyms

Synonyms Comment Organism
cyclodextrinase
-
Palaeococcus pacificus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
95
-
-
Palaeococcus pacificus

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
70 100 more than 50% activity between 70 and 100°C Palaeococcus pacificus

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
75 95 the enzyme retains more than 90% activity after 8 h at 75-85°C. At 95°C, the enzyme shows about 77%, 45%, 30% and less than 20% activity after 1 h, 2 h, 4 h and 8 h, respectively Palaeococcus pacificus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6
-
-
Palaeococcus pacificus

pH Range

pH Minimum pH Maximum Comment Organism
5 8 about 87% activity at pH 5.0, 100% activity at pH 6.0, about 75% activity at pH 7.0, about 50% activity at pH 8.0 Palaeococcus pacificus