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Literature summary for 3.2.1.55 extracted from

  • Kaji, A.; Yoshihara, O.
    Properties of purified alpha-L-arabinofuranosidase from Corticium rolfsii (1971), Biochim. Biophys. Acta, 250, 367-371.
    View publication on PubMed

Crystallization (Commentary)

Crystallization (Comment) Organism
-
Aspergillus niger

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.86
-
phenyl alpha-L-arabinofuranoside
-
Athelia rolfsii

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
beet arabinan + H2O Aspergillus niger
-
L-arabinose + ?
-
ir
beet arabinan + H2O Athelia rolfsii
-
L-arabinose + ?
-
ir

Organism

Organism UniProt Comment Textmining
Aspergillus niger
-
-
-
Athelia rolfsii
-
plant-pathogenic oxalate forming fungus, IFO 4878
-

Purification (Commentary)

Purification (Comment) Organism
-
Aspergillus niger
-
Athelia rolfsii

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
44
-
-
Athelia rolfsii

Storage Stability

Storage Stability Organism
30°C, purified enzyme at low pH values stable for 5 h to 20 days Athelia rolfsii

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1,5-L-arabinan + H2O
-
Aspergillus niger L-arabinose + ?
-
ir
1,5-L-arabinan + H2O
-
Athelia rolfsii L-arabinose + ?
-
ir
beet arabinan + H2O
-
Aspergillus niger L-arabinose + ?
-
ir
beet arabinan + H2O
-
Athelia rolfsii L-arabinose + ?
-
ir
phenyl alpha-L-arabinofuranoside + H2O
-
Aspergillus niger L-arabinose + phenol
-
ir
phenyl alpha-L-arabinofuranoside + H2O
-
Athelia rolfsii L-arabinose + phenol
-
ir

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
2.5
-
towards phenyl alpha-L-arabinofuranoside or beet arabinan Athelia rolfsii

pH Range

pH Minimum pH Maximum Comment Organism
1.5 3
-
Athelia rolfsii

pH Stability

pH Stability pH Stability Maximum Comment Organism
1.5 10 acid stable, even after storage at pH 2 and 5°C for 72 h, 83% enzymic activity remains Athelia rolfsii