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Literature summary for 3.2.1.55 extracted from

  • Hatanaka, H.; Imaoka, H.; Tajima, S.; Kasai, T.
    Purification and properties of an alpha-L-arabinofuranosidase from cotyledons of soybean seedlings (1991), Agric. Biol. Chem., 55, 2599-2605.
No PubMed abstract available
Search for more literature for 3.2.1.55

Activating Compound

Activating Compound Comment Organism Structure
D-galactono-1,4-lactone 0.5 mM, relative enzyme activity 102% Glycine max

Inhibitors

Inhibitors Comment Organism Structure
Ba2+
-
 Glycine max
Cu2+
-
 Glycine max
Cu2+
-
 Scopolia japonica
EDTA
-
 Glycine max
Hg2+
-
 Glycine max
L-arabino-gamma-lactone
-
 Glycine max

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.53
-
 4-nitrophenyl alpha-L-arabinofuranoside
-
 Glycine max

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ 0.5 mM CaCl2, relative enzyme activity 139% Glycine max
Co2+ 0.5 mM CoCl2, relative enzyme activity 111% Glycine max
Zn2+ activated by 0.5 mM ZnSO4, relative enzyme activity 180% Glycine max

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
87000
-
 SDS-PAGE Glycine max

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
beet arabinan + H2O Aspergillus niger
-
 L-arabinose + ?
-
 ir
beet arabinan + H2O Glycine max
-
 L-arabinose + ?
-
 ir
beet arabinan + H2O Scopolia japonica
-
 L-arabinose + ?
-
 ir

Organism

Organism UniProt Comment Textmining
Aspergillus niger
-
-
-
Daucus carota
-
 carrot
-
Glycine max
-
 soybean
-
Scopolia japonica
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Aspergillus niger
-
 Glycine max

Source Tissue

Source Tissue Comment Organism Textmining
callus
-
 Scopolia japonica
-
cotyledon
-
 Glycine max
-
seed
-
 Glycine max
-
seedling
-
 Glycine max
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
95.2
-
-
 Glycine max

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1,5-L-arabinan + H2O
-
 Aspergillus niger L-arabinose + ?
-
 ir
1,5-L-arabinan + H2O
-
 Glycine max L-arabinose + ?
-
 ir
4-nitrophenyl alpha-L-arabinofuranoside + H2O
-
 Glycine max 4-nitrophenol + alpha-L-arabinofuranose
-
 ?
beet arabinan + H2O
-
 Aspergillus niger L-arabinose + ?
-
 ir
beet arabinan + H2O
-
 Glycine max L-arabinose + ?
-
 ir
beet arabinan + H2O
-
 Scopolia japonica L-arabinose + ?
-
 ir
L-arabinan + H2O
-
 Aspergillus niger (1,5-alpha-L-arabinose)25 + ?
-
 ?
L-arabinan + H2O
-
 Daucus carota (1,5-alpha-L-arabinose)25 + ?
-
 ?
additional information
-
 Aspergillus niger ?
-
 ?
additional information enzyme does not degrade arabinogalactan or arabinoxylan, arabinose can not be hydrolyzed by the purified enzyme, beta-1,3 bonding arabinoside are not good substrates Glycine max ?
-
 ?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
45
-
-
 Glycine max

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
50
-
 stable up to Glycine max

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
4.8
-
-
 Glycine max
4.8
-
-
 Scopolia japonica