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Literature summary for 3.2.1.55 extracted from

  • Tsujibo, H.; Takada, C.; Wakamatsu, Y.; Kosaka, M.; Tsuji, A.; Miyamoto, K.; Inamori, Y.
    Cloning and expression of an alpha-L-arabinofuranosidase gene (stxIV) from Streptomyces thermoviolaceus OPC-520 (2002), Biosci. Biotechnol. Biochem., 66, 434-438.
    View publication on PubMed
Search for more literature for 3.2.1.55

Cloned(Commentary)

Cloned (Comment) Organism
abfB gene, nucleotide sequence determined Aspergillus nidulans
alpha-AF cDNA cloned from Trichoderma reesei RUT C-30 chromosomal DNA using PCR technique, trying to express this gene in the yeast Schizosaccharomyces pombe, expression in Saccharomyces cerevisiae Trichoderma reesei
cDNA clones isolated Hordeum vulgare
cloning and sequencing of the gene stxIV encoding alpha-L-arabinofuranosidase, expressed in Escherichia coli JM109 Streptomyces thermoviolaceus
gene cloning of 2 isoenzymes, PCR products subcloned into pCR2.1 plasmids Streptomyces chartreusis

Inhibitors

Inhibitors Comment Organism Structure
Cd2+
-
 Streptomyces thermoviolaceus
Co2+
-
 Streptomyces thermoviolaceus
Cu2+
-
 Streptomyces thermoviolaceus
Cu2+
-
 Thermoclostridium stercorarium
EDTA
-
 Aspergillus sojae
EDTA
-
 Streptomyces thermoviolaceus
Hg2+
-
 Aspergillus nidulans
Hg2+
-
 Streptomyces thermoviolaceus
Hg2+
-
 Thermoclostridium stercorarium
Ni2+
-
 Streptomyces thermoviolaceus
p-chloromercuribenzoate
-
 Streptomyces thermoviolaceus

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ weak stimulation of the enzyme activity Streptomyces thermoviolaceus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
35990
-
 calculated from amino acid sequence Streptomyces thermoviolaceus
37000
-
-
 Streptomyces chartreusis
37000
-
 SDS-PAGE Streptomyces thermoviolaceus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
oat spelt xylan + H2O Aspergillus niger
-
 L-arabinose + ?
-
 ?
oat spelt xylan + H2O Aspergillus sojae
-
 L-arabinose + ?
-
 ?
oat spelt xylan + H2O Trichoderma reesei
-
 L-arabinose + ?
-
 ?
oat spelt xylan + H2O Aspergillus awamori
-
 L-arabinose + ?
-
 ?
oat spelt xylan + H2O Thermoclostridium stercorarium
-
 L-arabinose + ?
-
 ?
oat spelt xylan + H2O Streptomyces thermoviolaceus
-
 L-arabinose + ?
-
 ?
oat spelt xylan + H2O Streptomyces thermoviolaceus OPC-520
-
 L-arabinose + ?
-
 ?

Organism

Organism UniProt Comment Textmining
Aspergillus awamori
-
-
-
Aspergillus nidulans
-
-
-
Aspergillus niger
-
-
-
Aspergillus sojae
-
-
-
Hordeum vulgare
-
-
-
Streptomyces chartreusis
-
 GS901
-
Streptomyces chartreusis GS901
-
 GS901
-
Streptomyces chattanoogensis
-
-
-
Streptomyces coelicolor O54161
-
-
Streptomyces lividans
-
 66strain 1326
-
Streptomyces lividans 66 1326
-
 66strain 1326
-
Streptomyces thermoviolaceus
-
 OPC-520
-
Streptomyces thermoviolaceus OPC-520
-
 OPC-520
-
Thermoclostridium stercorarium
-
-
-
Trichoderma reesei
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Hordeum vulgare
-
 Streptomyces chartreusis
recombinant enzyme, expressed in Escherichia coli Streptomyces thermoviolaceus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-arabinoxylan + H2O
-
 Aspergillus niger L-arabinose + ?
-
 ir
L-arabinoxylan + H2O
-
 Hordeum vulgare L-arabinose + ?
-
 ir
L-arabinoxylan + H2O
-
 Aspergillus sojae L-arabinose + ?
-
 ir
L-arabinoxylan + H2O
-
 Trichoderma reesei L-arabinose + ?
-
 ir
L-arabinoxylan + H2O
-
 Streptomyces lividans L-arabinose + ?
-
 ir
L-arabinoxylan + H2O
-
 Thermoclostridium stercorarium L-arabinose + ?
-
 ir
L-arabinoxylan + H2O
-
 Streptomyces chartreusis L-arabinose + ?
-
 ir
L-arabinoxylan + H2O
-
 Streptomyces thermoviolaceus L-arabinose + ?
-
 ir
L-arabinoxylan + H2O
-
 Streptomyces chartreusis GS901 L-arabinose + ?
-
 ir
L-arabinoxylan + H2O
-
 Streptomyces thermoviolaceus OPC-520 L-arabinose + ?
-
 ir
L-arabinoxylan + H2O
-
 Streptomyces lividans 66 1326 L-arabinose + ?
-
 ir
additional information
-
 Aspergillus niger ?
-
 ?
additional information
-
 Hordeum vulgare ?
-
 ?
additional information
-
 Aspergillus sojae ?
-
 ?
additional information
-
 Streptomyces lividans ?
-
 ?
additional information
-
 Streptomyces chartreusis ?
-
 ?
additional information no hydrolysis observed with birchwood xylan, linear 1,5-arabinan, arabinogalactan, gum arabic, p-nitrophenyl alpha-L-arabinopyranoside, p-nitrophenyl beta-D-xylopyranoside, p-nitrophenyl beta-D-galactopyranoside or p-nitrophenyl beta-D-glucopyranoside Streptomyces thermoviolaceus ?
-
 ?
additional information
-
 Streptomyces chartreusis GS901 ?
-
 ?
additional information no hydrolysis observed with birchwood xylan, linear 1,5-arabinan, arabinogalactan, gum arabic, p-nitrophenyl alpha-L-arabinopyranoside, p-nitrophenyl beta-D-xylopyranoside, p-nitrophenyl beta-D-galactopyranoside or p-nitrophenyl beta-D-glucopyranoside Streptomyces thermoviolaceus OPC-520 ?
-
 ?
additional information
-
 Streptomyces lividans 66 1326 ?
-
 ?
oat spelt xylan + H2O
-
 Aspergillus niger L-arabinose + ?
-
 ?
oat spelt xylan + H2O
-
 Aspergillus sojae L-arabinose + ?
-
 ?
oat spelt xylan + H2O
-
 Trichoderma reesei L-arabinose + ?
-
 ?
oat spelt xylan + H2O
-
 Aspergillus awamori L-arabinose + ?
-
 ?
oat spelt xylan + H2O
-
 Thermoclostridium stercorarium L-arabinose + ?
-
 ?
oat spelt xylan + H2O
-
 Streptomyces thermoviolaceus L-arabinose + ?
-
 ?
oat spelt xylan + H2O
-
 Streptomyces thermoviolaceus OPC-520 L-arabinose + ?
-
 ?
p-nitrophenyl-alpha-L-arabinofuranoside + H2O
-
 Aspergillus niger p-nitrophenol + L-arabinose
-
 ?
p-nitrophenyl-alpha-L-arabinofuranoside + H2O
-
 Hordeum vulgare p-nitrophenol + L-arabinose
-
 ?
p-nitrophenyl-alpha-L-arabinofuranoside + H2O
-
 Aspergillus sojae p-nitrophenol + L-arabinose
-
 ?
p-nitrophenyl-alpha-L-arabinofuranoside + H2O
-
 Trichoderma reesei p-nitrophenol + L-arabinose
-
 ?
p-nitrophenyl-alpha-L-arabinofuranoside + H2O
-
 Streptomyces lividans p-nitrophenol + L-arabinose
-
 ?
p-nitrophenyl-alpha-L-arabinofuranoside + H2O
-
 Thermoclostridium stercorarium p-nitrophenol + L-arabinose
-
 ?
p-nitrophenyl-alpha-L-arabinofuranoside + H2O
-
 Streptomyces chartreusis p-nitrophenol + L-arabinose
-
 ?
p-nitrophenyl-alpha-L-arabinofuranoside + H2O
-
 Streptomyces thermoviolaceus p-nitrophenol + L-arabinose
-
 ?
p-nitrophenyl-alpha-L-arabinofuranoside + H2O
-
 Streptomyces chartreusis GS901 p-nitrophenol + L-arabinose
-
 ?
p-nitrophenyl-alpha-L-arabinofuranoside + H2O
-
 Streptomyces thermoviolaceus OPC-520 p-nitrophenol + L-arabinose
-
 ?
p-nitrophenyl-alpha-L-arabinofuranoside + H2O
-
 Streptomyces lividans 66 1326 p-nitrophenol + L-arabinose
-
 ?
phenyl alpha-L-arabinofuranoside + H2O
-
 Aspergillus nidulans L-arabinose + phenol
-
 ir
phenyl alpha-L-arabinofuranoside + H2O
-
 Aspergillus niger L-arabinose + phenol
-
 ir
phenyl alpha-L-arabinofuranoside + H2O
-
 Hordeum vulgare L-arabinose + phenol
-
 ir
phenyl alpha-L-arabinofuranoside + H2O
-
 Aspergillus sojae L-arabinose + phenol
-
 ir
phenyl alpha-L-arabinofuranoside + H2O
-
 Trichoderma reesei L-arabinose + phenol
-
 ir
phenyl alpha-L-arabinofuranoside + H2O
-
 Streptomyces lividans L-arabinose + phenol
-
 ir
phenyl alpha-L-arabinofuranoside + H2O
-
 Aspergillus awamori L-arabinose + phenol
-
 ir
phenyl alpha-L-arabinofuranoside + H2O
-
 Thermoclostridium stercorarium L-arabinose + phenol
-
 ir
phenyl alpha-L-arabinofuranoside + H2O
-
 Streptomyces chartreusis L-arabinose + phenol
-
 ir
phenyl alpha-L-arabinofuranoside + H2O
-
 Streptomyces thermoviolaceus L-arabinose + phenol
-
 ir
phenyl alpha-L-arabinofuranoside + H2O
-
 Streptomyces chattanoogensis L-arabinose + phenol
-
 ir
phenyl alpha-L-arabinofuranoside + H2O
-
 Streptomyces coelicolor L-arabinose + phenol
-
 ir
phenyl alpha-L-arabinofuranoside + H2O
-
 Streptomyces chartreusis GS901 L-arabinose + phenol
-
 ir
phenyl alpha-L-arabinofuranoside + H2O
-
 Streptomyces thermoviolaceus OPC-520 L-arabinose + phenol
-
 ir
phenyl alpha-L-arabinofuranoside + H2O
-
 Streptomyces lividans 66 1326 L-arabinose + phenol
-
 ir

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
60
-
-
 Aspergillus niger
60
-
-
 Hordeum vulgare
60
-
-
 Aspergillus sojae
60
-
-
 Trichoderma reesei
60
-
-
 Streptomyces lividans
60
-
-
 Aspergillus awamori
60
-
-
 Streptomyces thermoviolaceus

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
50 80 stable up to 50°C, retains 50% original activity at 80°C Streptomyces thermoviolaceus
60
-
-
 Aspergillus nidulans
60
-
-
 Hordeum vulgare
60
-
 loses activity completely after 10 min Streptomyces lividans

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5
-
-
 Aspergillus niger
5
-
-
 Aspergillus sojae
5
-
-
 Aspergillus awamori
5
-
-
 Thermoclostridium stercorarium
5
-
-
 Streptomyces thermoviolaceus