Application | Comment | Organism |
---|---|---|
agriculture | bioconversion of lignocellulose material for the improvement of animal feedstock digestibility | Geobacillus stearothermophilus |
nutrition | - |
Bacillus subtilis |
nutrition | aromatic potential in wine, exploitation in flavor improvement and wine aromatization | Geobacillus stearothermophilus |
paper production | - |
Bacillus subtilis |
paper production | potential industrial use in biobleaching of paper pulp, bioconversion of lignocellulose material to fermentative products and for the improvement of animal feedstock digestibility | Geobacillus stearothermophilus |
Cloned (Comment) | Organism |
---|---|
cloning of the abfA T-6 gene, PCR, T7 polymerase expression vector pET9d, expressed in Escherichia coli BL21(DE3) | Geobacillus stearothermophilus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.1 | - |
2,5-dinitrophenyl beta-D-xylopyranoside | mutant E175A | Geobacillus stearothermophilus | |
0.9 | - |
3,4-dinitrophenyl beta-D-xylopyranoside | mutant E175A | Geobacillus stearothermophilus | |
4.4 | - |
3,4-dinitrophenyl beta-D-xylopyranoside | wild-type Abfa T-6 | Geobacillus stearothermophilus | |
5.3 | - |
2-nitrophenyl beta-D-xylopyranoside | mutant E175A | Geobacillus stearothermophilus | |
8 | - |
2,5-dinitrophenyl beta-D-xylopyranoside | wild-type Abfa T-6 | Geobacillus stearothermophilus | |
15.3 | - |
2-nitrophenyl beta-D-xylopyranoside | wild-type Abfa T-6 | Geobacillus stearothermophilus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Agrobacterium tumefaciens | - |
- |
- |
Bacillus subtilis | - |
- |
- |
Geobacillus stearothermophilus | - |
T-6 | - |
Geobacillus stearothermophilus | - |
mutant E175A | - |
Geobacillus stearothermophilus T-6 | - |
T-6 | - |
Halalkalibacterium halodurans | - |
- |
- |
Mesorhizobium loti | - |
- |
- |
Sinorhizobium meliloti | - |
- |
- |
Streptomyces coelicolor | - |
- |
- |
Streptomyces lividans | - |
66strain 1326 | - |
Streptomyces lividans 66 1326 | - |
66strain 1326 | - |
Purification (Comment) | Organism |
---|---|
recombinant enzyme, expressed in Escherichia coli BL21(DE3) | Geobacillus stearothermophilus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2,5-dinitrophenyl beta-D-xylopyranoside + H2O | - |
Geobacillus stearothermophilus | 2,5-dinitrophenol + beta-D-xylopyranose | - |
? | |
2,5-dinitrophenyl beta-D-xylopyranoside + H2O | - |
Geobacillus stearothermophilus T-6 | 2,5-dinitrophenol + beta-D-xylopyranose | - |
? | |
2-nitrophenyl beta-D-xylopyranoside + H2O | - |
Geobacillus stearothermophilus | 2-nitrophenol + beta-D-xylopyranose | - |
? | |
2-nitrophenyl beta-D-xylopyranoside + H2O | - |
Geobacillus stearothermophilus T-6 | 2-nitrophenol + beta-D-xylopyranose | - |
? | |
3,4-dinitrophenyl beta-D-xylopyranoside + H2O | - |
Geobacillus stearothermophilus | 3,4-dinitrophenol + beta-D-xylopyranose | - |
? | |
3,4-dinitrophenyl beta-D-xylopyranoside + H2O | - |
Geobacillus stearothermophilus T-6 | 3,4-dinitrophenol + beta-D-xylopyranose | - |
? | |
p-nitrophenyl-alpha-L-arabinofuranoside + H2O | - |
Bacillus subtilis | p-nitrophenol + L-arabinose | - |
? | |
p-nitrophenyl-alpha-L-arabinofuranoside + H2O | - |
Geobacillus stearothermophilus | p-nitrophenol + L-arabinose | - |
? | |
p-nitrophenyl-alpha-L-arabinofuranoside + H2O | - |
Streptomyces lividans | p-nitrophenol + L-arabinose | - |
? | |
p-nitrophenyl-alpha-L-arabinofuranoside + H2O | - |
Streptomyces lividans 66 1326 | p-nitrophenol + L-arabinose | - |
? | |
p-nitrophenyl-alpha-L-arabinofuranoside + H2O | - |
Geobacillus stearothermophilus T-6 | p-nitrophenol + L-arabinose | - |
? | |
phenyl alpha-L-arabinofuranoside + H2O | - |
Sinorhizobium meliloti | L-arabinose + phenol | - |
ir | |
phenyl alpha-L-arabinofuranoside + H2O | - |
Bacillus subtilis | L-arabinose + phenol | - |
ir | |
phenyl alpha-L-arabinofuranoside + H2O | - |
Geobacillus stearothermophilus | L-arabinose + phenol | - |
ir | |
phenyl alpha-L-arabinofuranoside + H2O | - |
Streptomyces coelicolor | L-arabinose + phenol | - |
ir | |
phenyl alpha-L-arabinofuranoside + H2O | - |
Agrobacterium tumefaciens | L-arabinose + phenol | - |
ir | |
phenyl alpha-L-arabinofuranoside + H2O | - |
Mesorhizobium loti | L-arabinose + phenol | - |
ir | |
phenyl alpha-L-arabinofuranoside + H2O | - |
Streptomyces lividans | L-arabinose + phenol | - |
ir | |
phenyl alpha-L-arabinofuranoside + H2O | - |
Halalkalibacterium halodurans | L-arabinose + phenol | - |
ir | |
phenyl alpha-L-arabinofuranoside + H2O | - |
Streptomyces lividans 66 1326 | L-arabinose + phenol | - |
ir | |
phenyl alpha-L-arabinofuranoside + H2O | - |
Geobacillus stearothermophilus T-6 | L-arabinose + phenol | - |
ir |