Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21 DE3 cells | Acetivibrio thermocellus |
Crystallization (Comment) | Organism |
---|---|
hanging-drop vapor diffusion | Acetivibrio thermocellus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0055 | - |
wheat arabinoxylan | - |
Acetivibrio thermocellus | |
0.25 | - |
p-nitrophenyl-alpha-L-arabinofuranoside | - |
Acetivibrio thermocellus | |
0.55 | - |
alpha-1,5-arabinotriose | - |
Acetivibrio thermocellus | |
0.95 | - |
alpha-1,3-arabinoxylobiose | - |
Acetivibrio thermocellus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Acetivibrio thermocellus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
gel filtration | Acetivibrio thermocellus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
alpha-1,3-arabinoxylobiose + H2O | - |
Acetivibrio thermocellus | ? | - |
? | |
alpha-1,5-arabinotriose + H2O | - |
Acetivibrio thermocellus | ? | - |
? | |
p-nitrophenyl-alpha-L-arabinofuranoside + H2O | - |
Acetivibrio thermocellus | p-nitrophenol + alpha-L-arabinofuranose | - |
? | |
wheat arabinoxylan + H2O | - |
Acetivibrio thermocellus | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
hexamer | anion exchange chromatography | Acetivibrio thermocellus |
Synonyms | Comment | Organism |
---|---|---|
alpha-L-arabinofuranosidase | - |
Acetivibrio thermocellus |
Araf51A | - |
Acetivibrio thermocellus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
82 | - |
with 1 mM p-nitrophenyl-alpha-L-arabinofuranoside as substrate | Acetivibrio thermocellus |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.07 | - |
wheat arabinoxylan | - |
Acetivibrio thermocellus | |
4.6 | - |
alpha-1,5-arabinotriose | - |
Acetivibrio thermocellus | |
8.6 | - |
alpha-1,3-arabinoxylobiose | - |
Acetivibrio thermocellus | |
103 | - |
p-nitrophenyl-alpha-L-arabinofuranoside | pH 7.0, 37°C | Acetivibrio thermocellus |