Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli | Thermomicrobia bacterium |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Ni2+ | 2 mM, 55% activity inhibition | Thermomicrobia bacterium | |
Zn2+ | 2 mM, 100% activity inhibition | Thermomicrobia bacterium |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.6 | - |
p-nitrophenyl-alpha-L-arabinofuranoside | - |
Thermomicrobia bacterium |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
additional information | no effect of Mg2+, Ca2+, Mn2+ at 2 mM on AraF | Thermomicrobia bacterium |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
350000 | - |
gel filtration | Thermomicrobia bacterium |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Thermomicrobia bacterium | - |
- |
- |
Purification (Comment) | Organism |
---|---|
column chromatography | Thermomicrobia bacterium |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.03 | - |
oat spelt xylan | Thermomicrobia bacterium |
0.06 | - |
arabinoxylan | Thermomicrobia bacterium |
0.5 | - |
debranched arabinan | Thermomicrobia bacterium |
0.6 | - |
branched arabinan | Thermomicrobia bacterium |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
arabinoxylan + H2O | - |
Thermomicrobia bacterium | ? | - |
? | |
branched arabinan + H2O | - |
Thermomicrobia bacterium | ? | - |
? | |
debranched arabinan + H2O | - |
Thermomicrobia bacterium | ? | - |
? | |
additional information | no activity on arabinogalactan and gum arabic | Thermomicrobia bacterium | ? | - |
? | |
oat spelt xylan + H2O | - |
Thermomicrobia bacterium | ? | - |
? | |
p-nitrophenyl-alpha-L-arabinofuranoside + H2O | - |
Thermomicrobia bacterium | p-nitrophenol + alpha-L-arabinofuranose | - |
? |
Synonyms | Comment | Organism |
---|---|---|
alpha-L-arabinofuranosidase | - |
Thermomicrobia bacterium |
AraF | - |
Thermomicrobia bacterium |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
70 | - |
- |
Thermomicrobia bacterium |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
70 | - |
92% residual activity after 1h | Thermomicrobia bacterium |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6 | - |
- |
Thermomicrobia bacterium |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
3.5 | 8 | more than 20% activity | Thermomicrobia bacterium |