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Literature summary for 3.2.1.55 extracted from
- Characterization of the arfA gene from Bacillus stearothermophilus No. 236 and its protein product, alpha-L-arabinofuranosidase (2004), J. Microbiol. Biotechnol., 14, 474-482.
No PubMed abstract available
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| beta-xylosidase | - |
Geobacillus stearothermophilus | |
| endoxylanase | - |
Geobacillus stearothermophilus |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expressed in Escherichia coli | Geobacillus stearothermophilus |
General Stability
| General Stability | Organism |
|---|---|
| about 6 h half-life of the enzyme at 60°C | Geobacillus stearothermophilus |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 60000 | - |
SDS-PAGE | Geobacillus stearothermophilus |
| 190000 | - |
gel filtration | Geobacillus stearothermophilus |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| arabinoxylan + H2O | Geobacillus stearothermophilus | - |
L-arabinose + D-xylose | - |
? |  |
| p-nitrophenyl-alpha-L-arabinofuranoside + H2O | Geobacillus stearothermophilus | - |
p-nitrophenol + alpha-L-arabinofuranose | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Geobacillus stearothermophilus | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| method not mentioned | Geobacillus stearothermophilus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| arabinoxylan + H2O | - |
Geobacillus stearothermophilus | L-arabinose + D-xylose | - |
? | |
| p-nitrophenyl-alpha-L-arabinofuranoside + H2O | - |
Geobacillus stearothermophilus | p-nitrophenol + alpha-L-arabinofuranose | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| alpha-L-arabinofuranosidase | - |
Geobacillus stearothermophilus |
| Arfase | - |
Geobacillus stearothermophilus |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 55 | - |
- |
Geobacillus stearothermophilus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6.5 | - |
- |
Geobacillus stearothermophilus |
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Release 2023.1 (January 2023)
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