Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 3.2.1.55 extracted from

  • Raweesri, P.; Riangrungrojana, P.; Pinphanichakarn, P.
    alpha-L-Arabinofuranosidase from Streptomyces sp. PC22: purification, characterization and its synergistic action with xylanolytic enzymes in the degradation of xylan and agricultural residues (2008), Biores. Technol., 99, 8981-8986.
    View publication on PubMed
Search for more literature for 3.2.1.55

Application

Application Comment Organism
agriculture simple and low cost saccharification of agricultural residues including waste can be performed under mild conditions and the reducing sugars obtained are useful for bioconversion to valueadded products Streptomyces sp. PC22

Inhibitors

Inhibitors Comment Organism Structure
Cu2+
-
 Streptomyces sp. PC22
Fe2+ concentration 1 mM, reduces the enzyme activity to less than 50% of that in the absence of the metal ion Streptomyces sp. PC22
Hg2+
-
 Streptomyces sp. PC22
Mn2+ concentration 1 mM, reduces the enzyme activity to less than 50% of that in the absence of the metal ion Streptomyces sp. PC22
additional information no effect with Ca2+ and Mg2+; no effect with iodoacetamide Streptomyces sp. PC22
N-bromosuccinimide enzyme completely inactivated Streptomyces sp. PC22
Zn2+ concentration 1 mM, reduces the enzyme activity to less than 50% of that in the absence of the metal ion Streptomyces sp. PC22

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.23
-
 p-nitrophenyl-alpha-L-arabinofuranoside pH 6.0 Streptomyces sp. PC22

Localization

Localization Comment Organism GeneOntology No. Textmining
extracellular
-
 Streptomyces sp. PC22
-
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
404000
-
 gel filtration, subunit mass of about 79000 Da, SDS-PAGE Streptomyces sp. PC22

Organism

Organism UniProt Comment Textmining
Streptomyces sp. PC22
-
-
-

Purification (Commentary)

Purification (Comment) Organism
precipitation followed by column chromatography Streptomyces sp. PC22

Source Tissue

Source Tissue Comment Organism Textmining
culture supernatant
-
 Streptomyces sp. PC22
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information arabinofuranosidase shows a mild but significant synergistic effect in combination with other xylanolytic enzymes, including xylanase, beta-xylosidase and acetyl esterase, on the degradation of oat-spelt xylan, corn cob and corn husk substrates with a 1.25, 1.32 and 1.21fold increase in the amount of reducing sugar released Streptomyces sp. PC22 ?
-
 ?
additional information no activity with p-nitrophenyl-beta-D-xylopyranoside Streptomyces sp. PC22 ?
-
 ?
p-nitrophenyl-alpha-L-arabinofuranoside + H2O
-
 Streptomyces sp. PC22 p-nitrophenol + L-arabinofuranose
-
 ?

Subunits

Subunits Comment Organism
More molecular weight 404000, subunit mass about 79000 Da, SDS-PAGE Streptomyces sp. PC22

Synonyms

Synonyms Comment Organism
AFase
-
 Streptomyces sp. PC22
alpha-L-arabinofuranosidase
-
 Streptomyces sp. PC22

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
65
-
-
 Streptomyces sp. PC22

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
70
-
 AFase retains over 50% activity at 70°C Streptomyces sp. PC22

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6
-
-
 Streptomyces sp. PC22

pH Range

pH Minimum pH Maximum Comment Organism
5.5 9 20% activity at pH 9.0 Streptomyces sp. PC22

pH Stability

pH Stability pH Stability Maximum Comment Organism
5.5 9 20% activity at pH 9.0 Streptomyces sp. PC22