Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21(DE3) cells | Bifidobacterium adolescentis |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
60000 | - |
x * 60000, recombinant isoform AXH-d3, SDS-PAGE | Bifidobacterium adolescentis |
63000 | - |
x * 63000, recombinant isoform AbfA, SDS-PAGE | Bifidobacterium adolescentis |
87000 | - |
x * 87000, recombinant isoform AbfB, SDS-PAGE | Bifidobacterium adolescentis |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
arabinoxylan oligosaccharides + H2O | Bifidobacterium adolescentis | - |
L-arabinose + ? | - |
? | |
barley arabinoxylan + H2O | Bifidobacterium adolescentis | on barley arabinoxylan-0.76 the combination of isoform AbfA and AXH-d3 cleaves 35% of the total arabinose | L-arabinose + ? | - |
? | |
rye arabinoxylan + H2O | Bifidobacterium adolescentis | on rye arabinoxylan-0.52 the combination of isoform AbfA and AXH-d3 cleaves 30% of the total arabinose | L-arabinose | - |
? | |
sugar beet arabinan + H2O | Bifidobacterium adolescentis | isoforms AbfB and AXH-d3 cleave small amounts of L-arabinose from sugar beet arabinan (4% and 2% of the total arabinoses, respectively) | L-arabinose + ? | - |
? | |
wheat arabinoxylan + H2O | Bifidobacterium adolescentis | recombinant isoform AbfA acts synergistically with isoforms AbfB and AXH-d3. In combination with AXH-d3, it releases 60% of L-arabinose from wheat arabinoxylan. Isoform AbfA does not act on di-linked arabinoses, but does release mono(2)-linked arabinoses present in wheat arabinoxylan-0.51 | L-arabinose + ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bifidobacterium adolescentis | - |
- |
- |
Purification (Comment) | Organism |
---|---|
HisTrap column chromatography | Bifidobacterium adolescentis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-nitrophenyl alpha-L-arabinofuranoside + H2O | - |
Bifidobacterium adolescentis | 4-nitrophenol + alpha-L-arabinofuranose | - |
? | |
4-nitrophenyl beta-D-xylopyranoside + H2O | substrate for isoform AbfA | Bifidobacterium adolescentis | 4-nitrophenol + beta-D-xylopyranose | - |
? | |
arabinoxylan oligosaccharides + H2O | - |
Bifidobacterium adolescentis | L-arabinose + ? | - |
? | |
barley arabinoxylan + H2O | on barley arabinoxylan-0.76 the combination of isoform AbfA and AXH-d3 cleaves 35% of the total arabinose | Bifidobacterium adolescentis | L-arabinose + ? | - |
? | |
additional information | isoform AbfB shows no activity with 4-nitrophenyl beta-D-xylopyranoside, isoforms AbfB and AXH-d3 are not active on debranched arabinan. All three isozymes, AfbA, AfbB and AXH-d3, lack activity against xyloglucan | Bifidobacterium adolescentis | ? | - |
? | |
rye arabinoxylan + H2O | on rye arabinoxylan-0.52 the combination of isoform AbfA and AXH-d3 cleaves 30% of the total arabinose | Bifidobacterium adolescentis | L-arabinose | - |
? | |
sugar beet arabinan + H2O | isoforms AbfB and AXH-d3 cleave small amounts of L-arabinose from sugar beet arabinan (4% and 2% of the total arabinoses, respectively) | Bifidobacterium adolescentis | L-arabinose + ? | - |
? | |
wheat arabinoxylan + H2O | recombinant isoform AbfA acts synergistically with isoforms AbfB and AXH-d3. In combination with AXH-d3, it releases 60% of L-arabinose from wheat arabinoxylan. Isoform AbfA does not act on di-linked arabinoses, but does release mono(2)-linked arabinoses present in wheat arabinoxylan-0.51 | Bifidobacterium adolescentis | L-arabinose + ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 60000, recombinant isoform AXH-d3, SDS-PAGE | Bifidobacterium adolescentis |
? | x * 63000, recombinant isoform AbfA, SDS-PAGE | Bifidobacterium adolescentis |
? | x * 87000, recombinant isoform AbfB, SDS-PAGE | Bifidobacterium adolescentis |
Synonyms | Comment | Organism |
---|---|---|
AbfA | recombinant isoform | Bifidobacterium adolescentis |
AbfB | recombinant isoform | Bifidobacterium adolescentis |
alpha-L-arabinofuranosidase | - |
Bifidobacterium adolescentis |
AXH-d3 | recombinant isoform | Bifidobacterium adolescentis |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
optimal temperature for the hydrolysis of 4-nitrophenyl alpha-L-arabinofuranoside is 50 °C for recombinant isoform AbfB | Bifidobacterium adolescentis |
50 | - |
optimal temperature for the hydrolysis of 4-nitrophenyl alpha-L-arabinofuranoside is 50 °C for recombinant isoform AbfA | Bifidobacterium adolescentis |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6 | - |
optimal pH value for the hydrolysis of 4-nitrophenyl alpha-L-arabinofuranoside for recombinant isoforms AbfA and AbfB | Bifidobacterium adolescentis |