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Literature summary for 3.2.1.55 extracted from

  • Kim, Y.A.; Yoon, K.H.
    Characterization of a Paenibacillus woosongensis beta-xylosidase/alpha-arabinofuranosidase produced by recombinant Escherichia coli (2010), J. Microbiol. Biotechnol., 20, 1711-1716.
    View publication on PubMed
Search for more literature for 3.2.1.55

Activating Compound

Activating Compound Comment Organism Structure
SDS SDS (5 mM) enhances the enzymatic activity for 4-nitrophenyl alpha-L-arabinofuranoside by 12% Paenibacillus woosongensis

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3) Codon Plus cells Paenibacillus woosongensis

Inhibitors

Inhibitors Comment Organism Structure
Cu2+ 88% residual activity at 5 mM Paenibacillus woosongensis
EDTA 68% residual activity at 5 mM Paenibacillus woosongensis
Fe2+ 12% residual activity at 5 mM Paenibacillus woosongensis
Fe3+ 49% residual activity at 5 mM Paenibacillus woosongensis

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ 106% activity at 5 mM Paenibacillus woosongensis
K+ 107% activity at 5 mM Paenibacillus woosongensis
Mg2+ 145% activity at 5 mM Paenibacillus woosongensis
Mn2+ 133% activity at 5 mM Paenibacillus woosongensis
Ni2+ 109% activity at 5 mM Paenibacillus woosongensis

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
52852
-
 x * 52852, calculated from amino acid sequence Paenibacillus woosongensis

Organism

Organism UniProt Comment Textmining
Paenibacillus woosongensis E7DXB6
-
-
Paenibacillus woosongensis KCTC 3953 (DSM 16971) E7DXB6
-
-

Purification (Commentary)

Purification (Comment) Organism
Ni-NTA column chromatography Paenibacillus woosongensis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-nitrophenyl alpha-L-arabinofuranoside + H2O
-
 Paenibacillus woosongensis 4-nitrophenol + alpha-L-arabinofuranose
-
 ?
4-nitrophenyl alpha-L-arabinofuranoside + H2O
-
 Paenibacillus woosongensis DSM 16971 4-nitrophenol + alpha-L-arabinofuranose
-
 ?
4-nitrophenyl alpha-L-arabinofuranoside + H2O
-
 Paenibacillus woosongensis KCTC 3953 (DSM 16971) 4-nitrophenol + alpha-L-arabinofuranose
-
 ?
additional information XylC does use 4-nitrophenyl beta-xylopyranoside as substrate with about 2fold higher specific activity than 4-nitrophenyl alpha-L-arabinofuranoside. The relative enzyme activity is approximately 52% for 4-nitrophenyl beta-xylopyranoside and 76% for 4-nitrophenyl alpha-L-arabinofuranoside at pH 8.0. The enzyme shows very low activity against 4-nitrophenyl alpha-L-arabinopyranoside and 4-nitrophenyl beta-D-galactopyranoside Paenibacillus woosongensis ?
-
 ?
additional information XylC does use 4-nitrophenyl beta-xylopyranoside as substrate with about 2fold higher specific activity than 4-nitrophenyl alpha-L-arabinofuranoside. The relative enzyme activity is approximately 52% for 4-nitrophenyl beta-xylopyranoside and 76% for 4-nitrophenyl alpha-L-arabinofuranoside at pH 8.0. The enzyme shows very low activity against 4-nitrophenyl alpha-L-arabinopyranoside and 4-nitrophenyl beta-D-galactopyranoside Paenibacillus woosongensis DSM 16971 ?
-
 ?
additional information XylC does use 4-nitrophenyl beta-xylopyranoside as substrate with about 2fold higher specific activity than 4-nitrophenyl alpha-L-arabinofuranoside. The relative enzyme activity is approximately 52% for 4-nitrophenyl beta-xylopyranoside and 76% for 4-nitrophenyl alpha-L-arabinofuranoside at pH 8.0. The enzyme shows very low activity against 4-nitrophenyl alpha-L-arabinopyranoside and 4-nitrophenyl beta-D-galactopyranoside Paenibacillus woosongensis KCTC 3953 (DSM 16971) ?
-
 ?

Subunits

Subunits Comment Organism
? x * 52852, calculated from amino acid sequence Paenibacillus woosongensis
? x * 53000-54000, estimated from SDS-PAGE Paenibacillus woosongensis

Synonyms

Synonyms Comment Organism
beta-xylosidase/alpha-arabinofuranosidase bifunctional enzyme Paenibacillus woosongensis
XylC bifunctional enzyme Paenibacillus woosongensis

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
50
-
-
 Paenibacillus woosongensis

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
30 45 the enzyme is stable up to 30°C for 3 h, and it retains 83% of its maximal activity at 40°C after 3 h of incubation. However, the stability of the enzyme decreases rapidly at temperature above 45°C Paenibacillus woosongensis

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.5
-
-
 Paenibacillus woosongensis

pH Range

pH Minimum pH Maximum Comment Organism
6 7 more than 75% activity between pH 6.0 and 7.0 Paenibacillus woosongensis