Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 3.2.1.55 extracted from

  • De La Mare, M.; Guais, O.; Bonnin, E.; Weber, J.; Francois, J.M.
    Molecular and biochemical characterization of three GH62 alpha-L-arabinofuranosidases from the soil deuteromycete Penicillium funiculosum (2013), Enzyme Microb. Technol., 53, 351-358.
    View publication on PubMed
Search for more literature for 3.2.1.55

Cloned(Commentary)

Cloned (Comment) Organism
isozymes PfABF62a, PfABF62b, and PfABF62c, DNA and amino acid sequence determination and analysis, recombinant expression of His6-tagged wild-type isozymes and isozyme pfabf62c mutant pfabf62cDELTACBM in Pichia pastoris strain GS115, subcloning in Escherichia coli Talaromyces funiculosus

Protein Variants

Protein Variants Comment Organism
additional information construction of an isozyme pfabf62c mutant pfabf62cDELTACBM truncated of the DNA region encoding canonical cellulose binding module, CBM1 Talaromyces funiculosus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.33
-
 rye arabinoxylan pH 4.0, 40°C, isozyme mutant pfabf62cDELTACBM Talaromyces funiculosus
0.47
-
 wheat arabinoxylan pH 4.0, 40°C, isozyme PfABF62b Talaromyces funiculosus
0.58
-
 rye arabinoxylan pH 4.0, 40°C, isozyme PfABF62c Talaromyces funiculosus
1
-
 wheat arabinoxylan pH 4.0, 40°C, isozyme PfABF62c Talaromyces funiculosus
1.4
-
 wheat arabinoxylan pH 4.0, 40°C, isozyme mutant pfabf62cDELTACBM Talaromyces funiculosus
2.7
-
 rye arabinoxylan pH 4.0, 40°C, isozyme PfABF62a Talaromyces funiculosus
8.2
-
 wheat arabinoxylan pH 4.0, 40°C, isozyme PfABF62a Talaromyces funiculosus

Localization

Localization Comment Organism GeneOntology No. Textmining
extracellular the isozymes are secreted Talaromyces funiculosus
-
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
35400
-
 x * 37100, about isozyme PfABF62a, sequence calculation, x * 35400, about isozyme PfABF62b, sequence calculation, x * 41300, about isozyme PfABF62c, sequence calculation, x * 37400, about isozyme mutant pfabf62cDELTACBM, sequence calculation Talaromyces funiculosus
37100
-
 x * 37100, about isozyme PfABF62a, sequence calculation, x * 35400, about isozyme PfABF62b, sequence calculation, x * 41300, about isozyme PfABF62c, sequence calculation, x * 37400, about isozyme mutant pfabf62cDELTACBM, sequence calculation Talaromyces funiculosus
37400
-
 x * 37100, about isozyme PfABF62a, sequence calculation, x * 35400, about isozyme PfABF62b, sequence calculation, x * 41300, about isozyme PfABF62c, sequence calculation, x * 37400, about isozyme mutant pfabf62cDELTACBM, sequence calculation Talaromyces funiculosus
41300
-
 x * 37100, about isozyme PfABF62a, sequence calculation, x * 35400, about isozyme PfABF62b, sequence calculation, x * 41300, about isozyme PfABF62c, sequence calculation, x * 37400, about isozyme mutant pfabf62cDELTACBM, sequence calculation Talaromyces funiculosus

Organism

Organism UniProt Comment Textmining
Talaromyces funiculosus
-
 three isozymes PfABF62a, PfABF62b, and PfABF62c
-

Purification (Commentary)

Purification (Comment) Organism
recombinant His6-tagged wild-type isozymes and isozyme pfabf62c mutant pfabf62cDELTACBM from Pichia pastoris strain GS115 by immobilized metal affinity chromatography Talaromyces funiculosus

Reaction

Reaction Comment Organism Reaction ID
1,5-alpha-L-arabinofuranohexaose + 5 H2O = 6 alpha-L-arabinofuranose mode of action of Penicillium funiculosum family 62 isozymes, overview Talaromyces funiculosus
a

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
arabinan + H2O
-
 Talaromyces funiculosus ?
-
 ?
additional information no activity with other polysaccharides or polymers than arabinan and arabinoxylan Talaromyces funiculosus ?
-
 ?
rye arabinoxylan + H2O enzymatic fingerprinting analysis by high performance anion exchange chromatogra-phy with pulsed amperometric detection Talaromyces funiculosus ?
-
 ?
wheat arabinoxylan + H2O soluble and insoluble wheat arabinoxylans, enzymatic fingerprinting analysis by high performance anion exchange chromatogra-phy with pulsed amperometric detection Talaromyces funiculosus ?
-
 ?

Subunits

Subunits Comment Organism
? x * 37100, about isozyme PfABF62a, sequence calculation, x * 35400, about isozyme PfABF62b, sequence calculation, x * 41300, about isozyme PfABF62c, sequence calculation, x * 37400, about isozyme mutant pfabf62cDELTACBM, sequence calculation Talaromyces funiculosus

Synonyms

Synonyms Comment Organism
alpha-L-Afase
-
 Talaromyces funiculosus
alpha-L-arabinofuranosidase
-
 Talaromyces funiculosus
PfABF62a
-
 Talaromyces funiculosus
PfABF62b
-
 Talaromyces funiculosus
PfABF62c
-
 Talaromyces funiculosus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
40
-
 isozymes PfABF62a and PfABF62b Talaromyces funiculosus
50
-
 isozyme PfABF62c Talaromyces funiculosus

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.8
-
 rye arabinoxylan pH 4.0, 40°C, isozyme mutant pfabf62cDELTACBM Talaromyces funiculosus
0.85
-
 wheat arabinoxylan pH 4.0, 40°C, isozyme mutant pfabf62cDELTACBM Talaromyces funiculosus
0.925
-
 rye arabinoxylan pH 4.0, 40°C, isozyme PfABF62a Talaromyces funiculosus
0.96
-
 wheat arabinoxylan pH 4.0, 40°C, isozyme PfABF62c Talaromyces funiculosus
0.99
-
 wheat arabinoxylan pH 4.0, 40°C, isozyme PfABF62a Talaromyces funiculosus
1.11
-
 rye arabinoxylan pH 4.0, 40°C, isozyme PfABF62c Talaromyces funiculosus
1.2
-
 wheat arabinoxylan pH 4.0, 40°C, isozyme PfABF62b Talaromyces funiculosus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
2.6
-
 isozyme PfABF62a Talaromyces funiculosus
2.8
-
 isozyme PfABF62c Talaromyces funiculosus
4.5
-
 isozyme PfABF62b Talaromyces funiculosus

pI Value

Organism Comment pI Value Maximum pI Value
Talaromyces funiculosus isozyme PfABF62a, sequence calculation
-
 4.66
Talaromyces funiculosus isozyme PfABF62c, sequence calculation
-
 4.7
Talaromyces funiculosus isozyme mutant pfabf62cDELTACBM, sequence calculation
-
 4.71
Talaromyces funiculosus isozyme PfABF62b, sequence calculation
-
 5.24

General Information

General Information Comment Organism
evolution the isozymes belong to the family 62 glycoside hydrolase, GH62, sequence comparisons, only isozyme PfABF62c is unique in bearing at its N-terminus a canonical family 1 carbohydrate-binding module of 37 amino acids length, which helps the protein to bind to microcrystalline cellulose Talaromyces funiculosus
physiological function the enzyme participates in the hydrolysis of arabinoxylans in corn/wheat fibers used in poultry feed Talaromyces funiculosus

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
0.121
-
 wheat arabinoxylan pH 4.0, 40°C, isozyme PfABF62a Talaromyces funiculosus
0.35
-
 rye arabinoxylan pH 4.0, 40°C, isozyme PfABF62a Talaromyces funiculosus
0.64
-
 wheat arabinoxylan pH 4.0, 40°C, isozyme mutant pfabf62cDELTACBM Talaromyces funiculosus
0.92
-
 wheat arabinoxylan pH 4.0, 40°C, isozyme PfABF62c Talaromyces funiculosus
1.91
-
 rye arabinoxylan pH 4.0, 40°C, isozyme PfABF62c Talaromyces funiculosus
2.425
-
 rye arabinoxylan pH 4.0, 40°C, isozyme mutant pfabf62cDELTACBM Talaromyces funiculosus
2.56
-
 wheat arabinoxylan pH 4.0, 40°C, isozyme PfABF62b Talaromyces funiculosus