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Literature summary for 3.2.1.55 extracted from
- A novel GH43 alpha-L-arabinofuranosidase of Penicillium chrysogenum that preferentially degrades single-substituted arabinosyl side chains in arabinan (2014), Enzyme Microb. Technol., 58-59, 80-86.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| gene afq1, semi-quantitative RT-PCR expression analysis, recombinant expression in and secretion from Escherichia coli | Talaromyces funiculosus |
| gene afs1, semi-quantitative RT-PCR expression analysis, recombinant expression in and secretion from Escherichia coli | Penicillium chrysogenum |
| gene Pcabf43A, semi-quantitative RT-PCR expression analysis | Talaromyces funiculosus |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
additional information | with L-arabinan, 0.5 mg/ml calculated as mM L-arabinofuranose released, pH 5.0, 50°C | Penicillium chrysogenum | |
| 1.8 | - |
L-arabinan | pH 5.0, 35°C | Talaromyces funiculosus |  |
| 11.5 | - |
L-arabinan | pH 5.0, 50°C | Talaromyces funiculosus | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| extracellular | the isozyme is secreted | Penicillium chrysogenum | - |
- |
| extracellular | the isozyme is secreted | Talaromyces funiculosus | - |
- |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 33148 | - |
x * 33148 , isozyme PcABF43A, sequence calcualtion | Talaromyces funiculosus |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Penicillium chrysogenum | B5MGR1 | inducible gene afs1 encoding isozyme AFS1 | - |
| Penicillium chrysogenum 31B | B5MGR1 | inducible gene afs1 encoding isozyme AFS1 | - |
| Talaromyces funiculosus | B5MGR0 | constitutive gene afq1 encoding isozyme AFQ1 | - |
| Talaromyces funiculosus | L0N6Q8 | constitutive isozyme PcABF43A | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| native extracellular enzyme 7.7fold from Penicillium funiculosum culture medium supernatant by ultrafiltration, ammonium sulfate fractionation, dialysis, two different steps of anion exchange chromatography, and hydrophobic interaction chromatography, to homogeneity | Talaromyces funiculosus |
| recombinant isozyme AFQ1 from Escherichia coli culture supernatant | Talaromyces funiculosus |
| recombinant isozyme AFS1 from Escherichia coli culture supernatant | Penicillium chrysogenum |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 1 | - |
purified enzyme, pH 5.0, 35°C, substrate L-arabinan | Talaromyces funiculosus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 1,5-alpha-L-arabinofuranobiose + H2O | - |
Talaromyces funiculosus | ? | - |
? | |
| 4-nitrophenyl alpha-L-arabinofuranoside + H2O | - |
Penicillium chrysogenum | 4-nitrophenol + L-arabinofuranose | - |
? | |
| 4-nitrophenyl alpha-L-arabinofuranoside + H2O | - |
Talaromyces funiculosus | 4-nitrophenol + L-arabinofuranose | - |
? | |
| 4-nitrophenyl alpha-L-arabinofuranoside + H2O | - |
Penicillium chrysogenum 31B | 4-nitrophenol + L-arabinofuranose | - |
? | |
| alpha-(1->5)-L-arabinofuranotriose + H2O | branched and unbranched, feruloylation of branched substrate strongly decreases the enzyme activity of isozyme rAFQ1 | Talaromyces funiculosus | ? | - |
? | |
| alpha-L-Araf-(1->3)-[alpha-L-Araf-(1->5)]-alpha-L-Araf + H2O | branched, the activity of isozyme rAFS1on feruloylated branched L-arabinofuranotriose is almost the same as its activity on branched substrate | Penicillium chrysogenum | ? | - |
? | |
| alpha-L-Araf-(1->3)-[alpha-L-Araf-(1->5)]-alpha-L-Araf + H2O | branched, the activity of isozyme rAFS1on feruloylated branched L-arabinofuranotriose is almost the same as its activity on branched substrate | Penicillium chrysogenum 31B | ? | - |
? | |
| L-arabinan + H2O | - |
Talaromyces funiculosus | ? | - |
? | |
| L-arabinan + H2O | isozyme PcABF43A hydrolyzes arabinosyl side chains linked to C-2 or C-3 of single-substituted arabinose residues in L-arabinan, mode of action, overview | Talaromyces funiculosus | ? | - |
? | |
| L-arabinan + H2O | mode of action, overview | Penicillium chrysogenum | ? | - |
? | |
| L-arabinan + H2O | mode of action, overview | Penicillium chrysogenum 31B | ? | - |
? | |
| additional information | activity of isozyme PcABF43A is strong and specific on sugar beet L-arabinan but weak on debranched arabinan andarabinoxylan. No or poor activity on 4-nitrophenyl alpha-L-arabinofuranoside, alpha-1,5-L-arabinooligosaccharides, and branched arabinotriose | Talaromyces funiculosus | ? | - |
? | |
| additional information | no activity with unbranched L-arabinofuranotriose and 1,5-alpha-L-arabinofuranobiose | Penicillium chrysogenum | ? | - |
? | |
| additional information | no activity with unbranched L-arabinofuranotriose and 1,5-alpha-L-arabinofuranobiose | Penicillium chrysogenum 31B | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 33148 , isozyme PcABF43A, sequence calcualtion | Talaromyces funiculosus |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| ABF | - |
Penicillium chrysogenum |
| ABF | - |
Talaromyces funiculosus |
| AFQ1 | - |
Talaromyces funiculosus |
| AFS1 | - |
Penicillium chrysogenum |
| alpha-L-Afase | - |
Penicillium chrysogenum |
| alpha-L-Afase | - |
Talaromyces funiculosus |
| alpha-L-arabinofuranosidase | - |
Penicillium chrysogenum |
| alpha-L-arabinofuranosidase | - |
Talaromyces funiculosus |
| PcABF43A | - |
Talaromyces funiculosus |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 35 | - |
isozyme PcABF43A | Talaromyces funiculosus |
| 50 | - |
isozyme AFQ1 | Talaromyces funiculosus |
| 50 | - |
isozyme AFS1 | Penicillium chrysogenum |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | - |
purified native enzyme, pH 5.0, 1 h, more than 90% of the initial activity remains | Talaromyces funiculosus |
| 40 | - |
purified native enzyme, pH 5.0, 1 h, loss of 70% activity | Talaromyces funiculosus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 5 | - |
isozyme AFQ1 | Talaromyces funiculosus |
| 5 | - |
isozyme AFS1 | Penicillium chrysogenum |
| 5 | - |
isozyme PcABF43A | Talaromyces funiculosus |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 4 | 9 | purified native enzyme, 16 h, more than 80% of the initial activity remains | Talaromyces funiculosus |
| 5 | - |
purified native enzyme, 30°C, 1 h, more than 90% of the initial activity remains | Talaromyces funiculosus |
Expression
| Organism | Comment | Expression |
|---|---|---|
| Talaromyces funiculosus | isozyme Pcabf43A gene is constitutively expressed in Penicillium chrysogenum 31B at a low level, although the expression is induced with pectic components such as L-arabinose, L-rhamnose, and D-galacturonic acid | up |
| Penicillium chrysogenum | slightly induced by L-rhamnose, D-galacturonic acid, rhamnogalacturonan, and polygalacturonic acid | up |
| Talaromyces funiculosus | slightly induced by L-rhamnose, D-galacturonic acid, rhamnogalacturonan, and polygalacturonic acid | up |
General Information
| General Information | Comment | Organism |
|---|---|---|
| evolution | isozyme AFQ1 belongs to the family 51 glycoside hydrolase, GH51 | Talaromyces funiculosus |
| evolution | isozyme AFS1 belongs to the family 54 glycoside hydrolase, GH54 | Penicillium chrysogenum |
| additional information | comparison of isozyme gene expression profiles | Penicillium chrysogenum |
| additional information | comparison of isozyme gene expression profiles | Talaromyces funiculosus |
| physiological function | isozyme AFQ1 plays a role of saccharification in the degradation of L-arabinan by this fungus | Talaromyces funiculosus |
| physiological function | isozyme PcABF43A together with isozyme AFS1 function as debranching enzymes in this fungus | Penicillium chrysogenum |
| physiological function | isozyme PcABF43A together with isozyme AFS1 function as debranching enzymes in this fungus | Talaromyces funiculosus |
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Release 2023.1 (January 2023)
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