Cloned (Comment) | Organism |
---|---|
expression of His6-tagged enzyme in Pichia pastoris strain X33 | Podospora anserina |
gene UM04309.1, expression of His6-tagged enzyme in Pichia pastoris strain X33 | Ustilago maydis |
Crystallization (Comment) | Organism |
---|---|
purified recombinant His-tagged enzyme free or complexed with cellotriose, method screening and optimization, hanging-drop vapor-diffusion method is chosen, mixing of equal volumes of 18 mg/ml protein in 50 mM sodium acetate, pH 5.0, and 50 mM NaCl, with reservoir solution containing 25% w/v PEG 4000, 0.2 M calcium chloride, and 0.1 M Tris, pH 8.5, 1 week, 12°C, crystal soaking in ligand solution, X-ray diffraction structure determination and analysis at 1.44-1.85 A resolution, molecular replacement method | Podospora anserina |
purified recombinant His-tagged enzyme free or complexed with sulfur-SAD or L-arabinose, method screening and optimization, hanging-drop vapor-diffusion method is chosen, mixing of equal volumes of 18 mg/ml protein in 50 mM sodium acetate, pH 5.0, and 50 mM NaCl, with reservoir solution containing 20% w/v PEG 3350 and 0.2 M sodium phosphate, 6 days, 12°C, soaking of crystals in ligand solution, X-ray diffraction structure determination and analysis at 1.0-1.75 A resolution, solved by the sulfur-SAD phasing method | Ustilago maydis |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
cellohexaose | - |
Podospora anserina | |
additional information | no inhibition by cellohexaose | Ustilago maydis |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | Michaelis-Menten kinetics | Podospora anserina | |
additional information | - |
additional information | Michaelis-Menten kinetics | Ustilago maydis | |
6.1 | - |
4-nitrophenyl-alpha-L-arabinofuranoside | pH 5.0, 37°C | Podospora anserina | |
7.5 | - |
4-nitrophenyl-alpha-L-arabinofuranoside | pH 5.0, 37°C | Ustilago maydis |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
67840 | - |
x * 67840, His-tagged enzyme, sequence calculation | Ustilago maydis |
99350 | - |
x * 99350, His-tagged enzyme, sequence calculation | Podospora anserina |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-arabinoxylan + H2O | Podospora anserina | the enzyme efficiently removes the alpha-L-arabinosyl substituents from arabinoxylan | ? | - |
? | |
L-arabinoxylan + H2O | Ustilago maydis | the enzyme efficiently removes the alpha-L-arabinosyl substituents from arabinoxylan | ? | - |
? | |
L-arabinoxylan + H2O | Ustilago maydis BRFM1093 | the enzyme efficiently removes the alpha-L-arabinosyl substituents from arabinoxylan | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Podospora anserina | E2GHW5 | - |
- |
Ustilago maydis | A0A0D1BZQ4 | gene UM04309.1 | - |
Ustilago maydis BRFM1093 | A0A0D1BZQ4 | gene UM04309.1 | - |
Posttranslational Modification | Comment | Organism |
---|---|---|
glycoprotein | N-deglycosylation by glycosidase Endo-Hf | Podospora anserina |
Purification (Comment) | Organism |
---|---|
recombinant His6-tagged enzyme from Pichia pastoris strain X33 | Podospora anserina |
recombinant His6-tagged enzyme from Pichia pastoris strain X33 | Ustilago maydis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-nitrophenyl alpha-L-arabinofuranoside + H2O | - |
Podospora anserina | 4-nitrophenol + L-arabinofuranose | - |
? | |
4-nitrophenyl alpha-L-arabinofuranoside + H2O | - |
Ustilago maydis | 4-nitrophenol + L-arabinofuranose | - |
? | |
4-nitrophenyl alpha-L-arabinofuranoside + H2O | - |
Ustilago maydis BRFM1093 | 4-nitrophenol + L-arabinofuranose | - |
? | |
L-arabinoxylan + H2O | the enzyme efficiently removes the alpha-L-arabinosyl substituents from arabinoxylan | Podospora anserina | ? | - |
? | |
L-arabinoxylan + H2O | the enzyme efficiently removes the alpha-L-arabinosyl substituents from arabinoxylan | Ustilago maydis | ? | - |
? | |
L-arabinoxylan + H2O | the enzyme efficiently removes the alpha-L-arabinosyl substituents from arabinoxylan | Ustilago maydis BRFM1093 | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 67840, His-tagged enzyme, sequence calculation | Ustilago maydis |
? | x * 99350, His-tagged enzyme, sequence calculation | Podospora anserina |
More | three-dimensional structure, overview | Podospora anserina |
More | three-dimensional structure, overview | Ustilago maydis |
Synonyms | Comment | Organism |
---|---|---|
PaAbf62A | - |
Podospora anserina |
UmAbf62A | - |
Ustilago maydis |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Podospora anserina |
37 | - |
assay at | Ustilago maydis |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.039 | - |
4-nitrophenyl-alpha-L-arabinofuranoside | pH 5.0, 37°C | Ustilago maydis | |
0.38 | - |
4-nitrophenyl-alpha-L-arabinofuranoside | pH 5.0, 37°C | Podospora anserina |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
5 | - |
assay at | Podospora anserina |
5 | - |
assay at | Ustilago maydis |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
3.4 | - |
cellohexaose | pH 5.0, 37°C | Podospora anserina |
General Information | Comment | Organism |
---|---|---|
evolution | the enzyme belongs to the family 62 glycoside hydrolase, GH62, phylogenetic analysis. The overall three-dimensional structure of PaAbf62A reveals a five-bladed beta-propeller fold that confirms its predicted classification into clan GH-F together with GH43 alpha-L-arabinofuranosidases, structure-function relationships within the GH62 family, overview | Podospora anserina |
evolution | the enzyme belongs to the family 62 glycoside hydrolase, GH62, phylogenetic analysis. The overall three-dimensional structure of PaAbf62A reveals a five-bladed beta-propeller fold that confirms its predicted classification into clan GH-F together with GH43 alpha-L-arabinofuranosidases, structure-function relationships within the GH62 family, overview | Ustilago maydis |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.005 | - |
4-nitrophenyl-alpha-L-arabinofuranoside | pH 5.0, 37°C | Ustilago maydis | |
0.062 | - |
4-nitrophenyl-alpha-L-arabinofuranoside | pH 5.0, 37°C | Podospora anserina |