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Literature summary for 3.2.1.55 extracted from
- Overexpression, purification and characterisation of homologous alpha-L-arabinofuranosidase and endo-1,4-beta-D-glucanase in Aspergillus vadensis (2014), J. Ind. Microbiol. Biotechnol., 14, 1697-1708.
No PubMed abstract available
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| gene abfB, DNA and amino acid sequence determination and analysis, recombinant extracellular expression of His-tagged enzyme in Aspergillus vadensis strain CBS 113365 using gpdA promoter from Aspergillus vadensis, method development and evaluation, overview | Aspergillus vadensis |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| extracellular | the enzyme is secreted | Aspergillus vadensis | - |
- |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Aspergillus vadensis | - |
gene abfB | - |
| Aspergillus vadensis CBS 137441 | - |
gene abfB | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant His-tagged enzyme 75fold from Aspergillus vadensis strain CBS 113365 culture supernatant | Aspergillus vadensis |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 4-methylumbelliferyl-alpha-L-arabinopyranoside + H2O | artificial | Aspergillus vadensis | 4-methylumbelliferol + alpha-L-arabinopyranose | - |
? |  |
| 4-methylumbelliferyl-alpha-L-arabinopyranoside + H2O | artificial | Aspergillus vadensis CBS 137441 | 4-methylumbelliferol + alpha-L-arabinopyranose | - |
? | |
| 4-nitrophenyl alpha-L-arabinofuranoside + H2O | best substrate | Aspergillus vadensis | 4-nitrophenol + L-arabinofuranose | - |
? | |
| 4-nitrophenyl alpha-L-arabinofuranoside + H2O | best substrate | Aspergillus vadensis CBS 137441 | 4-nitrophenol + L-arabinofuranose | - |
? | |
| debranched sugar beet arabinan + H2O | low activity | Aspergillus vadensis | ? | - |
? | |
| debranched sugar beet arabinan + H2O | low activity | Aspergillus vadensis CBS 137441 | ? | - |
? | |
| sugar beet arabinan + H2O | low activity | Aspergillus vadensis | ? | - |
? | |
| sugar beet arabinan + H2O | low activity | Aspergillus vadensis CBS 137441 | ? | - |
? | |
| wheat flour arabinoxylan + H2O | low activity | Aspergillus vadensis | ? | - |
? | |
| wheat flour arabinoxylan + H2O | low activity | Aspergillus vadensis CBS 137441 | ? | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| alpha-arabinofuranosidase B | - |
Aspergillus vadensis |
| alpha-L-arabinofuranosidase | - |
Aspergillus vadensis |
| AvAbfB | - |
Aspergillus vadensis |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 60 | - |
recombinant enzyme | Aspergillus vadensis |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 25 | 70 | activity range, recombinant enzyme, overview | Aspergillus vadensis |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 50 | - |
purified recombinant His-tagged enzyme, 15 min, stable up to | Aspergillus vadensis |
| 60 | - |
purified recombinant His-tagged enzyme, 15 min, 77% activity remaining | Aspergillus vadensis |
| 70 | - |
purified recombinant His-tagged enzyme, 15 min, inactivation | Aspergillus vadensis |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 3.5 | - |
recombinant enzyme | Aspergillus vadensis |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 1 | 6 | activity range, recombinant enzyme, overview | Aspergillus vadensis |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 2 | 9 | purified recombinant His-tagged enzyme, 40°C, 1 h, completely stable at | Aspergillus vadensis |
General Information
| General Information | Comment | Organism |
|---|---|---|
| evolution | the enzyme belongs to glycosyl hydrolase family 54, GH54 | Aspergillus vadensis |
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Release 2023.1 (January 2023)
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