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Literature summary for 3.2.1.55 extracted from

  • Yang, W.; Bai, Y.; Yang, P.; Luo, H.; Huang, H.; Meng, K.; Shi, P.; Wang, Y.; Yao, B.
    A novel bifunctional GH51 exo-alpha-L-arabinofuranosidase/endo-xylanase from Alicyclobacillus sp. A4 with significant biomass-degrading capacity (2015), Biotechnol. Biofuels, 8, 197.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.2.1.55

Application

Application Comment Organism
degradation Abf51A shows greater synergistic effect in combination with xylanase (2.92fold) on wheat arabinoxylan degradation than other reported enzymes, the amounts of arabinose, xylose, and xylobiose are all increased in comparison to that by the enzymes acting individually Alicyclobacillus sp.

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Alicyclobacillus sp.

Protein Variants

Protein Variants Comment Organism
E175Q inactive Alicyclobacillus sp.
E194Q inactive Alicyclobacillus sp.

Inhibitors

Inhibitors Comment Organism Structure
Ag+ 5 mM, 9.6% residual activity Alicyclobacillus sp.
Co2+ 5 mM, 25.4% residual activity Alicyclobacillus sp.
K+ 5 mM, 48% residual activity; 5 mM, 58% residual activity Alicyclobacillus sp.

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.46
-
 4-nitrophenyl alpha-L-arabinofuranoside pH 6.0, 60°C Alicyclobacillus sp.

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
57000
-
 x * 57000, SDS-PAGE Alicyclobacillus sp.

Organism

Organism UniProt Comment Textmining
Alicyclobacillus sp. A0A0P0ISK8
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-nitrophenyl alpha-L-arabinofuranoside + H2O
-
 Alicyclobacillus sp. 4-nitrophenol + L-arabinofuranose
-
 ?
debranched sugar beet arabinan + H2O
-
 Alicyclobacillus sp. L-arabinose + ?
-
 ?
additional information the enzyme exhibits an exo-type mode of action on polyarabinosides by catalyzing the cleavage of alpha-1,2- and alpha-1,3-linked arabinofuranose side chains in sugar beet arabinan and water-soluble wheat arabinoxylan and alpha-1,5-linked arabinofuranosidic bonds in debranched sugar beet arabinan. The enzyme additionally releases xylobiose and xylotriose from wheat arabinoxylan and is active on xylooligosaccharides (xylohexaose 1.2/mM/min, xylopentaose 6.9/mM/min, and xylotetraose 19.7/mM/min), however a lower level of activity Alicyclobacillus sp. ?
-
 ?
sugar beet arabinan + H2O
-
 Alicyclobacillus sp. L-arabinose + ?
-
 ?
wheat arabinoxylan + H2O
-
 Alicyclobacillus sp. L-arabinose + ?
-
 ?

Subunits

Subunits Comment Organism
? x * 57000, SDS-PAGE Alicyclobacillus sp.

Synonyms

Synonyms Comment Organism
Abf51A
-
 Alicyclobacillus sp.

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
60
-
-
 Alicyclobacillus sp.

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
65
-
 pH 4-11, stable for 12 h Alicyclobacillus sp.

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
23
-
 4-nitrophenyl alpha-L-arabinofuranoside pH 6.0, 60°C Alicyclobacillus sp.

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6
-
-
 Alicyclobacillus sp.

pH Stability

pH Stability pH Stability Maximum Comment Organism
4 11 65°C, stable for 12 h Alicyclobacillus sp.

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
50
-
 4-nitrophenyl alpha-L-arabinofuranoside pH 6.0, 60°C Alicyclobacillus sp.