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Literature summary for 3.2.1.57 extracted from

  • Okada, G.; Takayanagi, T.; Miyahara, S.; Sawai, T.
    An isomalto-dextranase accompanied by isopullulanase activity from Arthrobacter globiformis T6 (1988), Agric. Biol. Chem., 52, 829-836.
No PubMed abstract available

Inhibitors

Inhibitors Comment Organism Structure
Ag+ 5 mM, partial inhibition Arthrobacter globiformis
Fe3+ 5 mM completeinactivation Arthrobacter globiformis
Hg2+ 5 mM complete inactivation Arthrobacter globiformis
KMnO4 5 mM complete inactivation Arthrobacter globiformis
N-bromosuccinimide 5 mM complete inactivation Arthrobacter globiformis

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
pullulan + H2O Arthrobacter globiformis
-
isopanose + (Glc)6
-
?
pullulan + H2O Arthrobacter globiformis T6
-
isopanose + (Glc)6
-
?

Organism

Organism UniProt Comment Textmining
Arthrobacter globiformis
-
T6, single enzyme with G2-dextranase EC 3.2.1.94 and isopullanase activity
-

Oxidation Stability

Oxidation Stability Organism
photooxidation in presence of Rose Bengal Arthrobacter globiformis

Purification (Commentary)

Purification (Comment) Organism
-
Arthrobacter globiformis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
pullulan + H2O
-
Arthrobacter globiformis isopanose + (Glc)6
-
?
pullulan + H2O
-
Arthrobacter globiformis T6 isopanose + (Glc)6
-
?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
60
-
-
Arthrobacter globiformis

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
50
-
below completely stable Arthrobacter globiformis
70
-
10 min, 80% loss of activity Arthrobacter globiformis
80
-
inactivated Arthrobacter globiformis

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
4.8
-
-
Arthrobacter globiformis

pH Range

pH Minimum pH Maximum Comment Organism
2.8 8 4°C Arthrobacter globiformis
4.1 8 45°C Arthrobacter globiformis