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Literature summary for 3.2.1.58 extracted from
- Characterization of a new acid stable exo-beta-1,3-glucanase of Rhizoctonia solani and its action on microbial polysaccharides (2009), Int. J. Biol. Macromol., 44, 92-97.
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
additional information | Michaelis-Menten kinetics, overview | Rhizoctonia solani |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 62000 | - |
x * 62000 | Rhizoctonia solani |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| laminarin + H2O | Rhizoctonia solani | - |
? | - |
? |  |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Rhizoctonia solani | - |
isolated from a commercial source Kitarase-M | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| native enzyme 152fold by ammonium sulfate fractionation, ion exchange chromatography and gel filtration | Rhizoctonia solani |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.265 | - |
purified native enzyme | Rhizoctonia solani |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| laminarin + H2O | - |
Rhizoctonia solani | ? | - |
? | |
| laminarin + H2O | from Laminarium digitatum | Rhizoctonia solani | ? | - |
? | |
| laminaripentaose + H2O | - |
Rhizoctonia solani | D-glucose + ? | - |
? | |
| laminaritetraose + H2O | - |
Rhizoctonia solani | D-glucose + ? | - |
? | |
| laminaritriose + H2O | - |
Rhizoctonia solani | D-glucose + ? | - |
? | |
| additional information | the enzyme can hydrolyze laminaripentaose and laminaritetraose completely in 5 min to glucose and laminaribiose or laminaritriose, whereas overnight incubation is required for complete hydrolysis of laminaritriose, substrate specificity of the purified beta-1,3-glucanase, overview | Rhizoctonia solani | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 62000 | Rhizoctonia solani |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| exo-beta-1,3-glucanase | - |
Rhizoctonia solani |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 40 | - |
- |
Rhizoctonia solani |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | 60 | activity range, profile, overview | Rhizoctonia solani |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | 40 | purified enzyme, highly stable between for 1 h in citrate-phosphate buffer at pH 7.0 | Rhizoctonia solani |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 5 | - |
- |
Rhizoctonia solani |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 3 | 7 | activity range, profile, overview | Rhizoctonia solani |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 3 | 7 | purified native enzyme, highly stable | Rhizoctonia solani |
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