Cloned (Comment) | Organism |
---|---|
gene WsEXG1, cloned from from both the genomic DNA and cDNA, DNA and amino acid sequence determination and analysis, subcloning of a His6-tagged enzyme in Escherichia coli strain DH5alpha, functional overexpression in Yarrowia lipolytica strain Po1h | Cyberlindnera saturnus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
1,10-phenanthroline | 40.5% inhibition at 5 mM | Cyberlindnera saturnus | |
Ag+ | over 97% inhibition at 5 mM | Cyberlindnera saturnus | |
Co2+ | 76.5% inhibition at 5 mM | Cyberlindnera saturnus | |
Cu2+ | 87.7% inhibition at 5 mM | Cyberlindnera saturnus | |
EDTA | 99% inhibition at 5 mM | Cyberlindnera saturnus | |
EGTA | 94.5% inhibition at 5 mM | Cyberlindnera saturnus | |
Fe2+ | 18.8% inhibition at 5 mM | Cyberlindnera saturnus | |
Fe3+ | over 97% inhibition at 5 mM | Cyberlindnera saturnus | |
Hg2+ | over 97% inhibition at 5 mM | Cyberlindnera saturnus | |
iodoacetic acid | 74.4% inhibition at 5 mM | Cyberlindnera saturnus | |
Mn2+ | 87.5% inhibition at 5 mM | Cyberlindnera saturnus | |
Ni2+ | 83.5% inhibition at 5 mM | Cyberlindnera saturnus | |
PMSF | 24% inhibition at 5 mM | Cyberlindnera saturnus | |
SDS | 34% inhibition at 5 mM | Cyberlindnera saturnus | |
Zn2+ | 76% inhibition at 5 mM | Cyberlindnera saturnus |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ba2+ | activates 12% at 5 mM | Cyberlindnera saturnus | |
additional information | no effect by 5 mM of Mg2+, Ca2+, Na+, K+, or Li+ | Cyberlindnera saturnus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
46000 | - |
x * 46200, sequence calculation, x * 46000, recombinant enzyme, SDS-PAGE, x * 47500, native enzyme, SDS-PAGE | Cyberlindnera saturnus |
46200 | - |
x * 46200, sequence calculation, x * 46000, recombinant enzyme, SDS-PAGE, x * 47500, native enzyme, SDS-PAGE | Cyberlindnera saturnus |
47500 | - |
x * 46200, sequence calculation, x * 46000, recombinant enzyme, SDS-PAGE, x * 47500, native enzyme, SDS-PAGE | Cyberlindnera saturnus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Cyberlindnera saturnus | C3VMY4 | i.e. Hansenula saturnus, a marine yeast, strain WC91-2, gene WsEXG1 | - |
no activity in Yarrowia lipolytica | - |
- |
- |
no activity in Yarrowia lipolytica Po1h | - |
- |
- |
Purification (Comment) | Organism |
---|---|
recombinant His6-tagged enzyme 2.1fold from Yarrowia lipolytica strain Po1h to homogeneity | Cyberlindnera saturnus |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
62 | - |
crude enzyme from wild-type strain WC91-2 | Cyberlindnera saturnus |
249 | - |
crude recombinant enzyme from overexpressing Yarrowia lipolytica strain Po1h | Cyberlindnera saturnus |
511 | - |
purified recombinant enzyme | Cyberlindnera saturnus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
laminarin + H2O | active site glutamate residue | Cyberlindnera saturnus | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 46200, sequence calculation, x * 46000, recombinant enzyme, SDS-PAGE, x * 47500, native enzyme, SDS-PAGE | Cyberlindnera saturnus |
More | enzyme model with active site glutamate residue, overview | Cyberlindnera saturnus |
Synonyms | Comment | Organism |
---|---|---|
exo-beta-1,3-glucanase | - |
Cyberlindnera saturnus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
40 | - |
- |
Cyberlindnera saturnus |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | 55 | activity range | Cyberlindnera saturnus |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
40 | - |
purified recombinant enzyme, stable up to | Cyberlindnera saturnus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
5 | - |
- |
Cyberlindnera saturnus |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
3 | 9.5 | recombinant enzyme, 4°C, 2 h, stable | Cyberlindnera saturnus |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Cyberlindnera saturnus | sequence calculation | - |
4.5 |