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Literature summary for 3.2.1.6 extracted from
- Preliminary characterization of laminarinase from Trichoderma longibrachiatum (1987), Enzyme Microb. Technol., 9, 89-93.
No PubMed abstract available
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| D-glucose | 75% loss of activity by 2 mM | Trichoderma longibrachiatum |  |
| Hg2+ | 33% loss of activity by 0.5 mM | Trichoderma longibrachiatum | |
| KMnO4 | 56% loss of activity by 0.5 mM, complete inactivation by 2 mM | Trichoderma longibrachiatum | |
| Mn2+ | 50% loss of activity by 0.5 mM | Trichoderma longibrachiatum | |
| N-bromosuccinimide | 81% loss of activity by 0.5 mM, complete inactivation by 2 mM | Trichoderma longibrachiatum | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
additional information | - |
Trichoderma longibrachiatum |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| extracellular | - |
Trichoderma longibrachiatum | - |
- |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Trichoderma longibrachiatum | - |
- |
- |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| laminarin + H2O | - |
Trichoderma longibrachiatum | D-glucose + laminaribiose + laminaritriose + laminaritetraose + laminaripentaose + laminarihexaose | laminaridextrins + D-glucose | ? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| laminarinase | - |
Trichoderma longibrachiatum |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 50 | - |
- |
Trichoderma longibrachiatum |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | 80 | 90% of maximal activity at 30°C, 40% of maximal activity at 80°C | Trichoderma longibrachiatum |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | 60 | stable at 30°C, 25% of original activity lost after incubation at 40°C for 1 h, complete denaturation within 10 min at 60°C | Trichoderma longibrachiatum |
| 60 | - |
absence of substrate, 10 min, complete loss of activity | Trichoderma longibrachiatum |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 5 | - |
- |
Trichoderma longibrachiatum |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 3 | 9 | 4°C, 48 h stable with 5-20% loss of activity | Trichoderma longibrachiatum |
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