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Literature summary for 3.2.1.62 extracted from

  • Skovbjerg, H.; Noren, O.; Sjöström, H.; Danielsen, E.M.; Enevoldsen, B.S.
    Further characterization of intestinal lactase/phlorizin hydrolase (1982), Biochim. Biophys. Acta, 707, 89-97.
    View publication on PubMed

Inhibitors

Inhibitors Comment Organism Structure
phlorizin competitive inhibition of lactase activity Homo sapiens
phlorizin competitive inhibition of lactase activity, Ki: 0.37 mM Sus scrofa
Tris(hydroxymethyl)aminomethane
-
Homo sapiens
Tris(hydroxymethyl)aminomethane competitive inhibition of lactase activity, Ki: 5.1 mM Sus scrofa

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
5.1
-
4-O-beta-D-galactopyranosyl-D-glucopyranoside slightly higher catalytic efficiency than for the human enzyme Sus scrofa

Localization

Localization Comment Organism GeneOntology No. Textmining
microvillus
-
Homo sapiens 5902
-
microvillus
-
Sus scrofa 5902
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
160000
-
2 * 160000, SDS-PAGE Sus scrofa
320000
-
gel filtration, amphiphilic form Homo sapiens
320000
-
gel filtration, amphiphilic form Sus scrofa

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
4-O-beta-D-galactopyranosyl-D-glucopyranoside + H2O Homo sapiens
-
D-galactose + beta-D-glucose
-
?
4-O-beta-D-galactopyranosyl-D-glucopyranoside + H2O Sus scrofa two enzymatic activities, one for lactose- and one for phlorizin-and lactose-hydrolysis D-galactose + beta-D-glucose
-
?
4-O-beta-D-glucopyranosyl-D-glucose + H2O Homo sapiens cellobiose beta-D-glucose + D-glucose
-
?
4-O-beta-D-glucopyranosyl-D-glucose + H2O Sus scrofa cellobiose beta-D-glucose + D-glucose
-
?
6-O-beta-D-glucopyranosyl-D-glucose + H2O Sus scrofa low activity beta-D-glucose + D-glucose
-
?
6-O-beta-D-glucopyranosyl-D-glucose + H2O Homo sapiens gentiobiose beta-D-glucose + D-glucose
-
?
6-O-beta-D-glucopyranosyl-D-glucose + H2O Sus scrofa gentiobiose beta-D-glucose + D-glucose
-
?
cellotetraose + H2O Homo sapiens considerable activity ? + beta-D-glucose
-
?
cellotetraose + H2O Sus scrofa considerable activity ? + beta-D-glucose
-
?
cellotriose + H2O Homo sapiens considerable activity ? + beta-D-glucose
-
?
cellotriose + H2O Sus scrofa considerable activity ? + beta-D-glucose
-
?
cellulose + H2O Homo sapiens very low activity ? + beta-D-glucose
-
?
cellulose + H2O Sus scrofa very low activity ? + beta-D-glucose
-
?
phlorizin + H2O Homo sapiens
-
phloretin + beta-D-glucose
-
?
phlorizin + H2O Sus scrofa 5% of the activity of lactose hydrolysis phloretin + beta-D-glucose
-
?
phlorizin + H2O Sus scrofa hydrolysis located on a specific active site for phlorizin hydrolysis phloretin + beta-D-glucose
-
?

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-
Sus scrofa
-
-
-

Purification (Commentary)

Purification (Comment) Organism
to homogeneity, immunoadsorbent chromatography, amphiphilic form Sus scrofa

Source Tissue

Source Tissue Comment Organism Textmining
intestine small intestine Homo sapiens
-
intestine small intestine Sus scrofa
-
intestine proximal jejenum Sus scrofa
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2-naphthyl-beta-D-galactopyranoside + H2O very low activity Sus scrofa 2-naphthol + beta-D-galactopyranose
-
?
2-nitrophenyl beta-D-galactopyranoside + H2O
-
Homo sapiens 2-nitrophenol + beta-D-galactose
-
?
2-nitrophenyl beta-D-galactopyranoside + H2O lower activity than for 4-nitrophenyl-beta-D-galactopyranoside Sus scrofa 2-nitrophenol + beta-D-galactose
-
?
4-nitrophenyl beta-D-galactopyranoside + H2O
-
Homo sapiens 4-nitrophenol + beta-D-galactose
-
?
4-nitrophenyl beta-D-galactopyranoside + H2O
-
Sus scrofa 4-nitrophenol + beta-D-galactose
-
?
4-nitrophenyl beta-D-glucopyranoside + H2O
-
Homo sapiens 4-nitrophenol + beta-D-glucose
-
?
4-nitrophenyl beta-D-glucopyranoside + H2O
-
Sus scrofa 4-nitrophenol + beta-D-glucose
-
?
4-O-beta-D-galactopyranosyl-D-glucopyranoside + H2O
-
Homo sapiens D-galactose + beta-D-glucose
-
?
4-O-beta-D-galactopyranosyl-D-glucopyranoside + H2O two enzymatic activites, one for lactose- and one for phlorizin-and lactose-hydrolysis Sus scrofa D-galactose + beta-D-glucose
-
?
4-O-beta-D-galactopyranosyl-D-glucopyranoside + H2O two enzymatic activities, one for lactose- and one for phlorizin-and lactose-hydrolysis Sus scrofa D-galactose + beta-D-glucose
-
?
4-O-beta-D-glucopyranosyl-D-glucose + H2O cellobiose Homo sapiens beta-D-glucose + D-glucose
-
?
4-O-beta-D-glucopyranosyl-D-glucose + H2O cellobiose Sus scrofa beta-D-glucose + D-glucose
-
?
6-bromonaphthyl-beta-D-galactopyranoside very low activity Sus scrofa 6-bromonaphthol + beta-D-galactopyranose
-
?
6-O-beta-D-glucopyranosyl-D-glucose + H2O low activity Sus scrofa beta-D-glucose + D-glucose
-
?
6-O-beta-D-glucopyranosyl-D-glucose + H2O gentiobiose Homo sapiens beta-D-glucose + D-glucose
-
?
6-O-beta-D-glucopyranosyl-D-glucose + H2O gentiobiose Sus scrofa beta-D-glucose + D-glucose
-
?
cellotetraose + H2O considerable activity Homo sapiens ? + beta-D-glucose
-
?
cellotetraose + H2O considerable activity Sus scrofa ? + beta-D-glucose
-
?
cellotriose + H2O considerable activity Homo sapiens ? + beta-D-glucose
-
?
cellotriose + H2O considerable activity Sus scrofa ? + beta-D-glucose
-
?
cellulose + H2O very low activity Homo sapiens ? + beta-D-glucose
-
?
cellulose + H2O very low activity Sus scrofa ? + beta-D-glucose
-
?
phenyl-beta-D-galactopyranoside + H2O low activity Sus scrofa phenol + beta-D-galactopyranose
-
?
phlorizin + H2O
-
Homo sapiens phloretin + beta-D-glucose
-
?
phlorizin + H2O 5% of the activity of lactose hydrolysis Sus scrofa phloretin + beta-D-glucose
-
?
phlorizin + H2O hydrolysis located on a specific active site for phlorizin hydrolysis Sus scrofa phloretin + beta-D-glucose
-
?

Subunits

Subunits Comment Organism
dimer
-
Homo sapiens
dimer 2 * 160000, SDS-PAGE Sus scrofa

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
49
-
lactase activity faster destroyed than phlorizin activity Sus scrofa

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5.6 6.2
-
Sus scrofa

pH Stability

pH Stability pH Stability Maximum Comment Organism
3.2
-
little activity below Sus scrofa