Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Ba2+ | 1 mM reduce activity 15-28% | Daucus carota | |
Ba2+ | - |
Marchantia polymorpha | |
Cu2+ | 1 mM reduces activity 15-28% | Daucus carota | |
Cu2+ | reduces activity 20-53% | Marchantia polymorpha | |
Hg2+ | 1 mM reduces activity 15-28% | Daucus carota | |
Hg2+ | - |
Marchantia polymorpha | |
Mn2+ | - |
Marchantia polymorpha |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.032 | - |
(1,4-alpha-D-galacturonide)n | - |
Marchantia polymorpha | |
0.43 | - |
(1,4-alpha-D-galacturonide)n | - |
Daucus carota |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
48000 | - |
gel filtration | Daucus carota |
76000 | - |
gel filtration | Marchantia polymorpha |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
pectic acid + H2O | Daucus carota | - |
D-galacturonide + ? | - |
? | |
pectic acid + H2O | Marchantia polymorpha | - |
D-galacturonide + ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Daucus carota | - |
carrot | - |
Marchantia polymorpha | - |
liverwort, cell line HYH-2F | - |
Purification (Comment) | Organism |
---|---|
- |
Daucus carota |
- |
Marchantia polymorpha |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
root | - |
Daucus carota | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.08 | - |
- |
Marchantia polymorpha |
0.35 | - |
- |
Daucus carota |
Storage Stability | Organism |
---|---|
-20°C, 1 month | Marchantia polymorpha |
-20°C, 2 months | Daucus carota |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(1,4-alpha-D-galacturonide)n + H2O | - |
Daucus carota | D-galacturonate + digalacturonic acid + trigalacturonic acid | - |
? | |
(1,4-alpha-D-galacturonide)n + H2O | - |
Marchantia polymorpha | D-galacturonate + digalacturonic acid + trigalacturonic acid | - |
? | |
pectic acid + H2O | - |
Daucus carota | D-galacturonic acid + ? | - |
? | |
pectic acid + H2O | - |
Marchantia polymorpha | D-galacturonic acid + ? | - |
? | |
pectic acid + H2O | - |
Daucus carota | D-galacturonide + ? | - |
? | |
pectic acid + H2O | - |
Marchantia polymorpha | D-galacturonide + ? | - |
? | |
pectin + H2O | - |
Daucus carota | digalacturonate + D-galacturonic acid | - |
? | |
pectin + H2O | - |
Marchantia polymorpha | digalacturonate + D-galacturonic acid | - |
? |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
55 | - |
30 min barely inactivates enzyme, one-half of activity destroyed when heated to 65°C, completely destroyed at 80°C | Daucus carota |
55 | - |
30 min barely inactivates enzyme, one-half of activity destroyed when heated to 65°C, completely destroyed at 80°C | Marchantia polymorpha |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
3.6 | 3.8 | - |
Marchantia polymorpha |
4.8 | - |
- |
Daucus carota |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
3 | 6.5 | - |
Marchantia polymorpha |
3.5 | 7 | - |
Daucus carota |