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Literature summary for 3.2.1.67 extracted from

  • Konno, H.
    Galacturan 1,4-alpha-galacturonidase from carrot Daucus carota and liverwort Marchantia polymorpha (1988), Methods Enzymol., 161, 373-380.
No PubMed abstract available

Inhibitors

Inhibitors Comment Organism Structure
Ba2+ 1 mM reduce activity 15-28% Daucus carota
Ba2+
-
Marchantia polymorpha
Cu2+ 1 mM reduces activity 15-28% Daucus carota
Cu2+ reduces activity 20-53% Marchantia polymorpha
Hg2+ 1 mM reduces activity 15-28% Daucus carota
Hg2+
-
Marchantia polymorpha
Mn2+
-
Marchantia polymorpha

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.032
-
(1,4-alpha-D-galacturonide)n
-
Marchantia polymorpha
0.43
-
(1,4-alpha-D-galacturonide)n
-
Daucus carota

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
48000
-
gel filtration Daucus carota
76000
-
gel filtration Marchantia polymorpha

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
pectic acid + H2O Daucus carota
-
D-galacturonide + ?
-
?
pectic acid + H2O Marchantia polymorpha
-
D-galacturonide + ?
-
?

Organism

Organism UniProt Comment Textmining
Daucus carota
-
carrot
-
Marchantia polymorpha
-
liverwort, cell line HYH-2F
-

Purification (Commentary)

Purification (Comment) Organism
-
Daucus carota
-
Marchantia polymorpha

Source Tissue

Source Tissue Comment Organism Textmining
root
-
Daucus carota
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.08
-
-
Marchantia polymorpha
0.35
-
-
Daucus carota

Storage Stability

Storage Stability Organism
-20°C, 1 month Marchantia polymorpha
-20°C, 2 months Daucus carota

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(1,4-alpha-D-galacturonide)n + H2O
-
Daucus carota D-galacturonate + digalacturonic acid + trigalacturonic acid
-
?
(1,4-alpha-D-galacturonide)n + H2O
-
Marchantia polymorpha D-galacturonate + digalacturonic acid + trigalacturonic acid
-
?
pectic acid + H2O
-
Daucus carota D-galacturonic acid + ?
-
?
pectic acid + H2O
-
Marchantia polymorpha D-galacturonic acid + ?
-
?
pectic acid + H2O
-
Daucus carota D-galacturonide + ?
-
?
pectic acid + H2O
-
Marchantia polymorpha D-galacturonide + ?
-
?
pectin + H2O
-
Daucus carota digalacturonate + D-galacturonic acid
-
?
pectin + H2O
-
Marchantia polymorpha digalacturonate + D-galacturonic acid
-
?

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
55
-
30 min barely inactivates enzyme, one-half of activity destroyed when heated to 65°C, completely destroyed at 80°C Daucus carota
55
-
30 min barely inactivates enzyme, one-half of activity destroyed when heated to 65°C, completely destroyed at 80°C Marchantia polymorpha

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
3.6 3.8
-
Marchantia polymorpha
4.8
-
-
Daucus carota

pH Stability

pH Stability pH Stability Maximum Comment Organism
3 6.5
-
Marchantia polymorpha
3.5 7
-
Daucus carota