Literature summary for 3.2.1.67 extracted from

Amin, F.; Bhatti, H.N.; Bilal, M.; Asgher, M.
Purification, kinetic, and thermodynamic characteristics of an exo-polygalacturonase from Penicillium notatum with industrial perspective (2017), Appl. Biochem. Biotechnol., 183, 426-443 .

Search for more literature for 3.2.1.67

Activating Compound

Activating Compound	Comment	Organism	Structure
Triton X-100	about 115% activity at 1% (v/v)	Penicillium chrysogenum

Inhibitors

Inhibitors	Comment	Organism	Structure
Al3+	about 65% residual activity at 2 mM	Penicillium chrysogenum
Ca2+	about 60% residual activity at 2 mM	Penicillium chrysogenum
Cd2+	about 65% residual activity at 2 mM	Penicillium chrysogenum
Citric acid	about 90% residual activity at 1% (v/v)	Penicillium chrysogenum
Cr3+	about 63% residual activity at 2 mM	Penicillium chrysogenum
Cu2+	about 70% residual activity at 2 mM	Penicillium chrysogenum
EDTA	about 42% residual activity at 1% (v/v)	Penicillium chrysogenum
Hg2+	about 50% residual activity at 2 mM	Penicillium chrysogenum
Mg2+	about 65% residual activity at 2 mM	Penicillium chrysogenum
Ni2+	about 75% residual activity at 2 mM	Penicillium chrysogenum
Pb2+	about 75% residual activity at 2 mM	Penicillium chrysogenum
SDS	about 45% residual activity at 1% (w/v)	Penicillium chrysogenum
Sodium azide	-	Penicillium chrysogenum
Sr2+	about 90% residual activity at 2 mM	Penicillium chrysogenum
Tween 80	about 90% residual activity at 1% (v/v)	Penicillium chrysogenum
Urea	-	Penicillium chrysogenum
Zn2+	about 63% residual activity at 2 mM	Penicillium chrysogenum

Localization

Localization	Comment	Organism	GeneOntology No.	Textmining
extracellular	-	Penicillium chrysogenum	-	-

Metals/Ions

Metals/Ions	Comment	Organism	Structure
Co2+	about 110% activity at 2 mM	Penicillium chrysogenum
Mn2+	about 125% activity at 2 mM	Penicillium chrysogenum

Organism

Organism	UniProt	Comment	Textmining
Penicillium chrysogenum	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
ammonium sulfate precipitation, DEAE cellulose column chromatography, and Superdex G-75 gel filtration	Penicillium chrysogenum

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
polygalacturonic acid + H2O	-	Penicillium chrysogenum	galacturonic acid	-	?

Subunits

Subunits	Comment	Organism
?	x * 20000, isoform exo-PGII, SDS-PAGE	Penicillium chrysogenum
?	x * 85000, isoform exo-PGI, SDS-PAGE	Penicillium chrysogenum

Synonyms

Synonyms	Comment	Organism
exo-PGI	isoform	Penicillium chrysogenum
exo-PGII	isoform	Penicillium chrysogenum
exo-polygalacturonanase	-	Penicillium chrysogenum

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
50	-	a significant loss in enzymatic activity is observed at temperatures greater than 50°C	Penicillium chrysogenum

Temperature Stability [°C]

Temperature Stability Minimum [°C]	Temperature Stability Maximum [°C]	Comment	Organism
55	80	phase I of isoform exo-PGI shows half-lives of 6.83 and 2.39 min at 55°C and 80°C, respectively, whereas phase II of the enzyme exhibits a half-life of 63.57 and 22.72 min at 55°C and 80°C, respectively	Penicillium chrysogenum

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
6	-	-	Penicillium chrysogenum

pH Stability

pH Stability	pH Stability Maximum	Comment	Organism
4.5	6	the purified enzyme exhibits good stability in the pH range of 4.5-6.0 for 24 h	Penicillium chrysogenum

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Release 2023.1 (January 2023)