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Literature summary for 3.2.1.67 extracted from
- Purification and characterization of two inducible exopolygalacturonases from Aspergillus kawachii (2017), Biocatal. Agricult. Biotechnol., 10, 38-45 .
No PubMed abstract available
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Ca2+ | isoform exoPG2 shows 40% inhibition at 1 mM | Aspergillus luchuensis |  |
| Hg2+ | isoform exoPG1 shows 50% inhibition at 1 mM, isoform exoPG2 shows 97% inhibition at 1 mM | Aspergillus luchuensis | |
| Mn2+ | isoform exoPG1 shows 16% inhibition at 1 mM | Aspergillus luchuensis | |
| Zn2+ | isoform exoPG2 shows 15% inhibition at 1 mM | Aspergillus luchuensis | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Ca2+ | isoform exoPG1 shows 106% activity at 1 mM | Aspergillus luchuensis | |
| Mg2+ | isoform exoPG1 shows 114% activity at 1 mM | Aspergillus luchuensis | |
| additional information | Zn2+ and Co2+ have no effect on the catalysis of isoform exoPG1. Mg2+ and Mn2+ do not affect catalysis of isoform exoPG2 | Aspergillus luchuensis |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| pectin + H2O | Aspergillus luchuensis | 53% degree of methylation, marginal activity | galacturonic acid + ? | - |
? | |
| pectin + H2O | Aspergillus luchuensis IFO 4308 | 53% degree of methylation, marginal activity | galacturonic acid + ? | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Aspergillus luchuensis | - |
- |
- |
| Aspergillus luchuensis IFO 4308 | - |
- |
- |
Posttranslational Modification
| Posttranslational Modification | Comment | Organism |
|---|---|---|
| glycoprotein | - |
Aspergillus luchuensis |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| Q-Sepharose column chromatography, SP-Sepharose column chromatography, Sephadex G-25 gel filtration, and Sephacryl S-100 gel filtration | Aspergillus luchuensis |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| digalacturonic acid + H2O | complete hydrolysis | Aspergillus luchuensis | 2 galacturonic acid | - |
? | |
| digalacturonic acid + H2O | complete hydrolysis | Aspergillus luchuensis IFO 4308 | 2 galacturonic acid | - |
? | |
| pectin + H2O | 53% degree of methylation, marginal activity | Aspergillus luchuensis | galacturonic acid + ? | - |
? | |
| pectin + H2O | 53% degree of methylation, marginal activity | Aspergillus luchuensis IFO 4308 | galacturonic acid + ? | - |
? | |
| polygalacturonic acid + H2O | incomplete hydrolysis, after 24 h incubation about 50% of the substrate is hydrolyzed | Aspergillus luchuensis | galacturonic acid | - |
? | |
| polygalacturonic acid + H2O | incomplete hydrolysis, after 24 h incubation about 50% of the substrate is hydrolyzed | Aspergillus luchuensis IFO 4308 | galacturonic acid | - |
? | |
| trigalacturonic acid + H2O | complete hydrolysis | Aspergillus luchuensis | digalacturonic acid + galacturonic acid | - |
? | |
| trigalacturonic acid + H2O | complete hydrolysis | Aspergillus luchuensis IFO 4308 | digalacturonic acid + galacturonic acid | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 75000, isoform exoPG1, SDS-PAGE | Aspergillus luchuensis |
| ? | x * 80000, isoform exoPG2, SDS-PAGE | Aspergillus luchuensis |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| exoPG1 | isoform | Aspergillus luchuensis |
| exoPG2 | isoform | Aspergillus luchuensis |
| exopolygalacturonase | - |
Aspergillus luchuensis |
| exopolygalacturonase X | - |
Aspergillus luchuensis |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | 60 | the respective calculated isoform exoPG1 stabilities at pH 4.0 after 2 h incubation at 37, 50, and 60°C are 95%, 79%, and 54%. Under acidic conditions (pH 2.2), the residual activity after 2 h incubation at the same temperatures decreases to 48%, 36%, and 16%, respectively | Aspergillus luchuensis |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 3 | 4 | isoform exoPG1 | Aspergillus luchuensis |
| 4 | - |
isoform exoPG2 | Aspergillus luchuensis |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 2.2 | 5 | isoform exoPG1 shows 50% and 35% activity at pH 2.2 and 5.0, respectively | Aspergillus luchuensis |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 4 | 5 | the maximum pH stability is in the range 4.0-5.0 irrespective of the incubation temperature | Aspergillus luchuensis |
pI Value
| Organism | Comment | pI Value Maximum | pI Value |
|---|---|---|---|
| Aspergillus luchuensis | isoform exoPG1, isoelectric focusing | 4.7 | 4 |
| Aspergillus luchuensis | isoform exoPG2, isoelectric focusing | - |
4.55 |
IC50 Value
| IC50 Value | IC50 Value Maximum | Comment | Organism | Inhibitor | Structure |
|---|---|---|---|---|---|
| 1 | - |
at pH 4.0 and 37°C | Aspergillus luchuensis | Hg2+ | |
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Release 2023.1 (January 2023)
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