Activating Compound | Comment | Organism | Structure |
---|---|---|---|
2-mercaptoethanol | 116.31% activity at 5 mM | Zygoascus hellenicus | |
L-cysteine | 121.4% activity at 5 mM | Zygoascus hellenicus | |
phenylmethylsulfonyl fluoride | 113.77% activity at 5 mM | Zygoascus hellenicus | |
Tween 20 | 109.11% activity at 5 mM | Zygoascus hellenicus | |
Tween 80 | 115.4% activity at 5 mM | Zygoascus hellenicus | |
Urea | 110.38% activity at 5 mM | Zygoascus hellenicus |
Application | Comment | Organism |
---|---|---|
food industry | the enzyme is applied in the juice clarification of tangerine, orange, grapefruit, and apple | Zygoascus hellenicus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Al3+ | 84.27% residual activity at 10 mM | Zygoascus hellenicus | |
Ba2+ | 85.24% residual activity at 10 mM | Zygoascus hellenicus | |
Co2+ | 87.23% residual activity at 10 mM | Zygoascus hellenicus | |
Fe2+ | 90.07% residual activity at 10 mM | Zygoascus hellenicus | |
GSH | 83.26% residual activity at 10 mM | Zygoascus hellenicus | |
Hg2+ | 31.41% residual activity at 10 mM | Zygoascus hellenicus | |
Pb2+ | 87.53% residual activity at 10 mM | Zygoascus hellenicus | |
phenylmethylsulfonyl fluoride | 81.4% residual activity at 10 mM | Zygoascus hellenicus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
extracellular | - |
Zygoascus hellenicus | - |
- |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Cu2+ | 140.11% activity at 10 mM | Zygoascus hellenicus | |
K+ | 117.79% activity at 10 mM | Zygoascus hellenicus | |
Mg2+ | 113.53% activity at 10 mM | Zygoascus hellenicus | |
Na+ | 108.74% activity at 10 mM | Zygoascus hellenicus | |
Zn2+ | 112.44% activity at 10 mM | Zygoascus hellenicus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Zygoascus hellenicus | - |
- |
- |
Zygoascus hellenicus V25 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
ammonium sulfate precipitation, DEAE cellulose chromatography, and Sephadex G-100 gel filtration | Zygoascus hellenicus |
Source Tissue | Comment | Organism | Textmining |
---|
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
citrus pectin DE 30% + H2O | 100% activity | Zygoascus hellenicus | galacturonic acid | - |
? | |
citrus pectin DE 30% + H2O | 100% activity | Zygoascus hellenicus V25 | galacturonic acid | - |
? | |
citrus pectin DE 72% + H2O | 58.19% activity compared to citrus pectin DE 30% | Zygoascus hellenicus | galacturonic acid | - |
? | |
citrus pectin DE 72% + H2O | 58.19% activity compared to citrus pectin DE 30% | Zygoascus hellenicus V25 | galacturonic acid | - |
? | |
citrus pectin DE 85% + H2O | 7.18% activity compared to citrus pectin DE 30% | Zygoascus hellenicus | galacturonic acid | - |
? | |
citrus pectin DE 85% + H2O | 7.18% activity compared to citrus pectin DE 30% | Zygoascus hellenicus V25 | galacturonic acid | - |
? | |
additional information | no activity with xylan and carboxymethyl cellulose | Zygoascus hellenicus | ? | - |
? | |
additional information | no activity with xylan and carboxymethyl cellulose | Zygoascus hellenicus V25 | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 75280, calculated from amino acid sequence | Zygoascus hellenicus |
Synonyms | Comment | Organism |
---|---|---|
exo-polygalacturonanase | - |
Zygoascus hellenicus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
60 | - |
- |
Zygoascus hellenicus |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
35 | 70 | the enzyme shows more than 50% activity between 35 and 70°C | Zygoascus hellenicus |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | 50 | the purified enzyme is stable between 30 and 50°C, retaining more than 90% of its activity after 4 h, but the thermal stability of the enzyme obviously declines at 60-70°C | Zygoascus hellenicus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
5 | - |
- |
Zygoascus hellenicus |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
3 | 7 | the enzyme shows more than 70% activity at pH 3.0-7.0 | Zygoascus hellenicus |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
3 | 11 | the enzyme retains more than 85% of its activity at pH 3.0-11.0 in 4 h | Zygoascus hellenicus |