Cloned (Comment) | Organism |
---|---|
DNA and amino acid sequence determination and analysis, phylogenetic tree | Bacillus sp. (in: Bacteria) |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Cu2+ | complete inhibition at 0.5 mM | Bacillus sp. (in: Bacteria) | |
Fe3+ | 59% inhibition at 0.5 mM, 90% at 5 mM | Bacillus sp. (in: Bacteria) | |
Hg2+ | complete inhibition at 0.5 mM | Bacillus sp. (in: Bacteria) | |
additional information | no inhibition by EDTA, citrate, maltose, and alpha-cyclodextrin | Bacillus sp. (in: Bacteria) | |
phenylmethanesulfonyl fluoride | 9% inhibition at 5 mM | Bacillus sp. (in: Bacteria) | |
SDS | 77% inhibition at 30 mM | Bacillus sp. (in: Bacteria) | |
Sn2+ | 28% inhibition at 0.5 mM, 73% at 5 mM | Bacillus sp. (in: Bacteria) | |
Urea | 82% inhibition at 2 M | Bacillus sp. (in: Bacteria) | |
Zn2+ | 64% inhibition at 0.5 mM, 88% at 5 mM | Bacillus sp. (in: Bacteria) |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | - |
Bacillus sp. (in: Bacteria) |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | activates 10% at 0.5 mM, and 25% at 5 mM, Ca2+ highly stabilizes the enzyme at 80°C | Bacillus sp. (in: Bacteria) | |
Co2+ | activates 12% at 5 mM | Bacillus sp. (in: Bacteria) | |
Mn2+ | activates 28% at 0.5 mM, and 41% at 5 mM | Bacillus sp. (in: Bacteria) | |
additional information | poor effects by Na+, K+, Mg2+, Li+, and EDTA at 0.5-5 mM. Mn2+ and Mg2+ do not stabilize the enzyme at 70°C and 80°C | Bacillus sp. (in: Bacteria) |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
100000 | - |
gel filtration | Bacillus sp. (in: Bacteria) |
101155 | - |
1 * 100000, SDS-PAGE, 1 * 101155, sequence calculation | Bacillus sp. (in: Bacteria) |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
amylopectin + H2O | Bacillus sp. (in: Bacteria) | - |
amylose + linear maltooligosaccharides | - |
? | |
amylopectin + H2O | Bacillus sp. (in: Bacteria) CICIM 304 | - |
amylose + linear maltooligosaccharides | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bacillus sp. (in: Bacteria) | L0C9D3 | isolated by screening a soil sample collected from a hot spring of Tengchong volcano in Yunnan, China | - |
Bacillus sp. (in: Bacteria) CICIM 304 | L0C9D3 | isolated by screening a soil sample collected from a hot spring of Tengchong volcano in Yunnan, China | - |
Purification (Comment) | Organism |
---|---|
native extracellular enzyme 369fold from culture supernatant by ultrafiltration, ammonium sulfate fractionation, gel filtration, two different steps of anion exchange chromatography, and dialysis, to homogeneity | Bacillus sp. (in: Bacteria) |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
6535 U/mg, measuring 0.01 increase in absorbance at 600 nm per h at 50°C, pH 6.0, per mg protein | Bacillus sp. (in: Bacteria) |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
amylopectin + H2O | - |
Bacillus sp. (in: Bacteria) | amylose + linear maltooligosaccharides | - |
? | |
amylopectin + H2O | - |
Bacillus sp. (in: Bacteria) CICIM 304 | amylose + linear maltooligosaccharides | - |
? | |
glycogen + H2O | - |
Bacillus sp. (in: Bacteria) | ? | - |
? | |
glycogen + H2O | - |
Bacillus sp. (in: Bacteria) CICIM 304 | ? | - |
? | |
maize starch + H2O | - |
Bacillus sp. (in: Bacteria) | ? | - |
? | |
maltodextrin + H2O | - |
Bacillus sp. (in: Bacteria) | ? | - |
? | |
maltodextrin + H2O | - |
Bacillus sp. (in: Bacteria) CICIM 304 | ? | - |
? | |
additional information | pullulan is a poor substrate | Bacillus sp. (in: Bacteria) | ? | - |
? | |
additional information | pullulan is a poor substrate | Bacillus sp. (in: Bacteria) CICIM 304 | ? | - |
? | |
soluble starch + H2O | - |
Bacillus sp. (in: Bacteria) | ? | - |
? | |
soluble starch + H2O | - |
Bacillus sp. (in: Bacteria) CICIM 304 | ? | - |
? | |
sticky rice starch + H2O | - |
Bacillus sp. (in: Bacteria) | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 100000, SDS-PAGE, 1 * 101155, sequence calculation | Bacillus sp. (in: Bacteria) |
Synonyms | Comment | Organism |
---|---|---|
glycogen-6-glucanogydrolase | - |
Bacillus sp. (in: Bacteria) |
IAM | - |
Bacillus sp. (in: Bacteria) |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
70 | - |
recombinant enzyme | Bacillus sp. (in: Bacteria) |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | 70 | purified native enzyme, pH 6.0, 1 h, over 70% activity remaining | Bacillus sp. (in: Bacteria) |
80 | - |
purified native enzyme, pH 6.0, 1 h, inactivation | Bacillus sp. (in: Bacteria) |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6.5 | - |
broad optimum, profile overview | Bacillus sp. (in: Bacteria) |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
5.5 | 9 | over 90% of maximal activity within this range, profile overview | Bacillus sp. (in: Bacteria) |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
4.5 | 9 | purified native enzyme, 50°C, 1 h, over 70% activity remaining | Bacillus sp. (in: Bacteria) |
General Information | Comment | Organism |
---|---|---|
evolution | the enzyme harbors a carbohydrate-binding module family 48 (CBM48) domain | Bacillus sp. (in: Bacteria) |
physiological function | isoamylase catalyzes hydrolysis of alpha-D-(1,6)-glucosidic branch linkages in amylopectin and glycogen to release amylose and linear maltooligosaccharides | Bacillus sp. (in: Bacteria) |