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Literature summary for 3.2.1.7 extracted from

  • Kang, S.I.; Chang, Y.J.; Oh, S.J.; Kim, S.I.
    Purification and properties of an endo-inulinase from an Arthrobacter sp. (1998), Biotechnol. Lett., 20, 983-986.
No PubMed abstract available
Search for more literature for 3.2.1.7

Inhibitors

Inhibitors Comment Organism Structure
EDTA 1 mM, 41% inactivation Arthrobacter sp.
Fe3+ 1 mM, 15% inactivation Arthrobacter sp.
Hg2+ 1 mM, 33% inactivation Arthrobacter sp.
L-Cys 1 mM, 6% inactivation Arthrobacter sp.

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.7
-
 inulin
-
 Arthrobacter sp.

Localization

Localization Comment Organism GeneOntology No. Textmining
extracellular
-
 Arthrobacter sp.
-
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
75000
-
 x * 75000, SDS-PAGE Arthrobacter sp.

Organism

Organism UniProt Comment Textmining
Arthrobacter sp.
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Arthrobacter sp.

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
53
-
-
 Arthrobacter sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
inulin + H2O
-
 Arthrobacter sp. oligofructosides mainly inulotriose, inulotetraose and inulopentaose ?

Subunits

Subunits Comment Organism
? x * 75000, SDS-PAGE Arthrobacter sp.

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
50
-
-
 Arthrobacter sp.

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
30 50 30°C: about 45% of maximal activity, 50°C: optimum, no activity at 60°C Arthrobacter sp.

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
30
-
 pH 7.5, 10 min, stable Arthrobacter sp.
40
-
 pH 7.5, 10 min, about 10% loss of activity Arthrobacter sp.
50
-
 pH 7.5, 10 min, about 60% loss of activity Arthrobacter sp.
60
-
 pH 7.5, 10 min, complete inactivation Arthrobacter sp.

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
-
 Arthrobacter sp.

pH Range

pH Minimum pH Maximum Comment Organism
5 10 pH 5.0: about 55% of maximal activity, pH 10.0: about 40% of maximal activity Arthrobacter sp.

pH Stability

pH Stability pH Stability Maximum Comment Organism
4
-
 4°C, 24 h, complete loss of activity Arthrobacter sp.
5
-
 4°C, 24 h, about 10% loss of activity Arthrobacter sp.
6 10.5 4°C, 24 h, stable Arthrobacter sp.