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Literature summary for 3.2.1.7 extracted from
- Optimization for high-level expression of the Pichia guilliermondii recombinant inulinase in Pichia pastoris and characterization of the recombinant inulinase (2009), Process Biochem., 44, 1335-1339.
No PubMed abstract available
Application
| Application | Comment | Organism |
|---|---|---|
| synthesis | high-level expression in Pichia pastoris leads to production of enzyme at 286.8 U/ml and 8873 U/mg | Meyerozyma guilliermondii |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression in Pichia pastoris | Meyerozyma guilliermondii |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Ag+ | 1 mM, 20% residual activity | Meyerozyma guilliermondii |  |
| Hg2+ | 1 mM, 25% residual activity | Meyerozyma guilliermondii | |
| Mg2+ | 1 mM, 80% residual activity | Meyerozyma guilliermondii | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Ca2+ | 1 mM, 35% activation | Meyerozyma guilliermondii | |
| Co2+ | 1 mM, 20% activation | Meyerozyma guilliermondii | |
| Cu2+ | 1 mM, 30% activation | Meyerozyma guilliermondii | |
| Fe2+ | 1 mM, 35% activation | Meyerozyma guilliermondii | |
| Fe3+ | 1 mM, 20% activation | Meyerozyma guilliermondii | |
| K+ | 1 mM, 35% activation | Meyerozyma guilliermondii | |
| Li+ | 1 mM, 35% activation | Meyerozyma guilliermondii | |
| Mn2+ | 1 mM, 35% activation | Meyerozyma guilliermondii | |
| Na+ | 1 mM, 35% activation | Meyerozyma guilliermondii | |
| Zn2+ | 1 mM, 40% activation | Meyerozyma guilliermondii | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 57600 | - |
x * 57600, SDS-PAGE | Meyerozyma guilliermondii |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Meyerozyma guilliermondii | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant enzyme | Meyerozyma guilliermondii |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 57600, SDS-PAGE | Meyerozyma guilliermondii |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 60 | - |
- |
Meyerozyma guilliermondii |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 60 | - |
stable up to, rapid inactivation above | Meyerozyma guilliermondii |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6 | - |
- |
Meyerozyma guilliermondii |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 6 | 7 | - |
Meyerozyma guilliermondii |
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Release 2023.1 (January 2023)
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