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Literature summary for 3.2.1.73 extracted from

  • Luo, H.; Yang, J.; Yang, P.; Li, J.; Huang, H.; Shi, P.; Bai, Y.; Wang, Y.; Fan, Y.; Yao, B.
    Gene cloning and expression of a new acidic family 7 endo-beta-1,3-1,4-glucanase from the acidophilic fungus Bispora sp. MEY-1 (2010), Appl. Microbiol. Biotechnol., 85, 1015-1023.
    View publication on PubMed

Application

Application Comment Organism
synthesis over-expression in Pichia pastoris, with a yield of about 1000 U/ml in a 3.7 l fermentor Bispora sp.

Cloned(Commentary)

Cloned (Comment) Organism
expression in Pichia pastoris Bispora sp.

General Stability

General Stability Organism
enzyme is highly resistant to both pepsin and trypsin Bispora sp.

Inhibitors

Inhibitors Comment Organism Structure
Ag+ strong inhibition Bispora sp.
EDTA strong inhibition Bispora sp.
Hg2+ strong inhibition Bispora sp.
Sodium dodecyl sulfate strong inhibition Bispora sp.

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
45170
-
x * 45170, calculated, x * 60000-80000, SDS-PAGE of recombinant enzyme, x * 48000, SDS-PAGE after Endo H treatment Bispora sp.
48000
-
x * 45170, calculated, x * 60000-80000, SDS-PAGE of recombinant enzyme, x * 48000, SDS-PAGE after Endo H treatment Bispora sp.

Organism

Organism UniProt Comment Textmining
Bispora sp. D2DRB6
-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
no glycoprotein five potential N-glycosylation site Asn-Xaa-Thr/Ser-Zaa, where Zaa is not Pro, are found. Endo H treatment decreases molecular mass from 60000-80000 to 48000, SDS-PAGE Bispora sp.
proteolytic modification presence of an N-terminal signal peptide at residues 1-9 Bispora sp.

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2740
-
substrate lichenan, pH 5.0, 60°C Bispora sp.
4040
-
substrtae beta-D-glucan, pH 5.0, 60°C Bispora sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
beta-D-glucan + H2O source: barley, best substrate Bispora sp. ? hydrolysis products from barley beta-glucan are 6.8 micromol/ml glucose and 16.3 micromol/ml cellobiose ?
carboxymethyl cellulose + H2O 9.8% of the activity with beta-D-glucan Bispora sp. ?
-
?
lichenan + H2O 72% of the activity with beta-D-glucan Bispora sp. ? hydrolysis products from lichenan are 9.1 micromol/ml glucose and 9.9 micromol/ml cellobiose, and 0.45 micromol/ml cellotetraose ?
additional information poor substrates: laminarin, oat spelt xylan. No substrate: avicel Bispora sp. ?
-
?

Subunits

Subunits Comment Organism
? x * 45170, calculated, x * 60000-80000, SDS-PAGE of recombinant enzyme, x * 48000, SDS-PAGE after Endo H treatment Bispora sp.

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
60
-
-
Bispora sp.

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
60
-
stable for at least 1 h Bispora sp.
70
-
5 min, 48% residual activity, 60 min, 30% residual activity Bispora sp.

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
1.5
-
three activity peaks at pH 1.5, 3.5, and 5.0. At pH 1.5, 61% of the activity at pH 5.0 Bispora sp.
3.5
-
three activity peaks at pH 1.5, 3.5, and 5.0. At pH 3.5, 91% of the activity at pH 5.0 Bispora sp.
5
-
three activity peaks at pH 1.5, 3.5, and 5.0. Maximum activity at pH 5.0 Bispora sp.

pH Stability

pH Stability pH Stability Maximum Comment Organism
1 8 37°C, 1 h, more than 85% of maximum activity Bispora sp.