Application | Comment | Organism |
---|---|---|
synthesis | over-expression in Pichia pastoris, with a yield of about 1000 U/ml in a 3.7 l fermentor | Bispora sp. |
Cloned (Comment) | Organism |
---|---|
expression in Pichia pastoris | Bispora sp. |
General Stability | Organism |
---|---|
enzyme is highly resistant to both pepsin and trypsin | Bispora sp. |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Ag+ | strong inhibition | Bispora sp. | |
EDTA | strong inhibition | Bispora sp. | |
Hg2+ | strong inhibition | Bispora sp. | |
Sodium dodecyl sulfate | strong inhibition | Bispora sp. |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
45170 | - |
x * 45170, calculated, x * 60000-80000, SDS-PAGE of recombinant enzyme, x * 48000, SDS-PAGE after Endo H treatment | Bispora sp. |
48000 | - |
x * 45170, calculated, x * 60000-80000, SDS-PAGE of recombinant enzyme, x * 48000, SDS-PAGE after Endo H treatment | Bispora sp. |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bispora sp. | D2DRB6 | - |
- |
Posttranslational Modification | Comment | Organism |
---|---|---|
no glycoprotein | five potential N-glycosylation site Asn-Xaa-Thr/Ser-Zaa, where Zaa is not Pro, are found. Endo H treatment decreases molecular mass from 60000-80000 to 48000, SDS-PAGE | Bispora sp. |
proteolytic modification | presence of an N-terminal signal peptide at residues 1-9 | Bispora sp. |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
2740 | - |
substrate lichenan, pH 5.0, 60°C | Bispora sp. |
4040 | - |
substrtae beta-D-glucan, pH 5.0, 60°C | Bispora sp. |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
beta-D-glucan + H2O | source: barley, best substrate | Bispora sp. | ? | hydrolysis products from barley beta-glucan are 6.8 micromol/ml glucose and 16.3 micromol/ml cellobiose | ? | |
carboxymethyl cellulose + H2O | 9.8% of the activity with beta-D-glucan | Bispora sp. | ? | - |
? | |
lichenan + H2O | 72% of the activity with beta-D-glucan | Bispora sp. | ? | hydrolysis products from lichenan are 9.1 micromol/ml glucose and 9.9 micromol/ml cellobiose, and 0.45 micromol/ml cellotetraose | ? | |
additional information | poor substrates: laminarin, oat spelt xylan. No substrate: avicel | Bispora sp. | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 45170, calculated, x * 60000-80000, SDS-PAGE of recombinant enzyme, x * 48000, SDS-PAGE after Endo H treatment | Bispora sp. |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
60 | - |
- |
Bispora sp. |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
60 | - |
stable for at least 1 h | Bispora sp. |
70 | - |
5 min, 48% residual activity, 60 min, 30% residual activity | Bispora sp. |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
1.5 | - |
three activity peaks at pH 1.5, 3.5, and 5.0. At pH 1.5, 61% of the activity at pH 5.0 | Bispora sp. |
3.5 | - |
three activity peaks at pH 1.5, 3.5, and 5.0. At pH 3.5, 91% of the activity at pH 5.0 | Bispora sp. |
5 | - |
three activity peaks at pH 1.5, 3.5, and 5.0. Maximum activity at pH 5.0 | Bispora sp. |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
1 | 8 | 37°C, 1 h, more than 85% of maximum activity | Bispora sp. |