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Literature summary for 3.2.1.74 extracted from

  • Zverlov, V.; Mahr, S.; Riedel, K.; Bronnenmeier, K.
    Properties and gene structure of a bifunctional celluloytic enzyme (CelA) from the extreme thermophile 'Anaerocellum thermophilum' with separate glycosyl hydrolase family 9 and 48 catalytic domains (1998), Microbiology, 144, 457-465.
    View publication on PubMed
Search for more literature for 3.2.1.74

Cloned(Commentary)

Cloned (Comment) Organism
Escherichia coli Caldicellulosiruptor bescii

Localization

Localization Comment Organism GeneOntology No. Textmining
extracellular
-
 Caldicellulosiruptor bescii
-
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
23000
-
 23000, SDS-PAGE Caldicellulosiruptor bescii

Organism

Organism UniProt Comment Textmining
Caldicellulosiruptor bescii
-
-
-

Purification (Commentary)

Purification (Comment) Organism
4.8fold Caldicellulosiruptor bescii

Source Tissue

Source Tissue Comment Organism Textmining
culture medium
-
 Caldicellulosiruptor bescii
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.285
-
-
 Caldicellulosiruptor bescii

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
Avicel + H2O
-
 Caldicellulosiruptor bescii D-glucose + cellotetraose + cellotriose + cellobiose
-
 ?
cellodextrins + H2O
-
 Caldicellulosiruptor bescii D-glucose + cellotriose + cellobiose
-
 ?

Subunits

Subunits Comment Organism
? 23000, SDS-PAGE Caldicellulosiruptor bescii

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
85
-
 avicelase activity Caldicellulosiruptor bescii
95 100 carboxymethyl cellulase activity Caldicellulosiruptor bescii

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5 6
-
 Caldicellulosiruptor bescii