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Literature summary for 3.2.1.78 extracted from
- A highly thermostable endo-(1,4)-beta-mannanase from the marine bacterium Rhodothermus marinus (2000), Appl. Microbiol. Biotechnol., 53, 715-721.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
- |
Rhodothermus marinus |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 113000 | - |
x * 113000, deduced from gene sequence, upon expression in Escherichia coli, two fragments of 45000 and 50000 Da | Rhodothermus marinus |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Rhodothermus marinus | P49425 | ATCC 43812 | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| carob-galactomannan + H2O | i.e. locust bean gum | Rhodothermus marinus | ? | - |
? |  |
| guar gum + H2O | - |
Rhodothermus marinus | ? | - |
? | |
| additional information | requires at least five sugar moieties for effective catalytic activity, no substrate: yeast mannan | Rhodothermus marinus | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 113000, deduced from gene sequence, upon expression in Escherichia coli, two fragments of 45000 and 50000 Da | Rhodothermus marinus |
| More | C-terminal domain of 550 amino acid residues with homology to glycosidase family 26 | Rhodothermus marinus |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 85 | - |
- |
Rhodothermus marinus |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 70 | - |
1 h, 70% residual activity, truncated protein fragments produced in Escherichia coli, 1 h, 87% residual activity, protein derived from Rhodothermus marinus | Rhodothermus marinus |
| 90 | - |
1 h, 25% residual activity, truncated protein fragments produced in Escherichia coli | Rhodothermus marinus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 5.4 | - |
- |
Rhodothermus marinus |
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Release 2023.1 (January 2023)
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