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Literature summary for 3.2.1.78 extracted from

  • Setati, M.E.; Ademark, P.; van Zyl, W.H.; Hahn-Hagerdal, B.; Stalbrand, H.
    Expression of the Aspergillus aculeatus endo-beta-1,4-mannanase encoding gene (man1) in Saccharomyces cerevisiae and characterization of the recombinant enzyme (2001), Protein Expr. Purif., 21, 105-114.
    View publication on PubMed
Search for more literature for 3.2.1.78

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
45000
-
 x * 50000, recombinant protein, x * 45000, native protein, SDS-PAGE Aspergillus aculeatus
50000
-
 x * 50000, recombinant protein, x * 45000, native protein, SDS-PAGE Aspergillus aculeatus

Organism

Organism UniProt Comment Textmining
Aspergillus aculeatus Q00012 MRC 11624
-
Aspergillus aculeatus MRC 11624 Q00012 MRC 11624
-

Posttranslational Modification

Posttranslational Modification Comment Organism
glycoprotein treatment with PNGaseF results in a shift to 41000 Da, both for native and recombinant protein Aspergillus aculeatus

Purification (Commentary)

Purification (Comment) Organism
both native protein and expressed in Saccharomyces cerevisiae Aspergillus aculeatus

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
64.6
-
 pH 5.0, 50°C, recombinant protein Aspergillus aculeatus
264.1
-
 pH 5.0, 50°C, native protein Aspergillus aculeatus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ivory nut mannan + H2O
-
 Aspergillus aculeatus ?
-
 ?
ivory nut mannan + H2O
-
 Aspergillus aculeatus MRC 11624 ?
-
 ?

Subunits

Subunits Comment Organism
? x * 50000, recombinant protein, x * 45000, native protein, SDS-PAGE Aspergillus aculeatus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
50
-
-
 Aspergillus aculeatus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
3
-
-
 Aspergillus aculeatus