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Literature summary for 3.2.1.78 extracted from
- LeMAN4 endo-beta-mannanase from ripe tomato fruit can act as a mannan transglycosylase or hydrolase (2006), Planta, 224, 1091-1102.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| NaCl | 0.3 M | Solanum lycopersicum |  |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expressed in Escherichia coli | Solanum lycopersicum |
Crystallization (Commentary)
| Crystallization (Comment) | Organism |
|---|---|
| recombinant enzyme | Solanum lycopersicum |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| NaCl | 1.3 M | Solanum lycopersicum | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 9 | - |
Manbeta(1-4)Manbeta(1-4)Man | - |
Solanum lycopersicum | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| cell wall | - |
Solanum lycopersicum | 5618 | - |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 39000 | - |
SDS-PAGE | Solanum lycopersicum |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Solanum lycopersicum | - |
tomato | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| salt extraction, dialysis, cation exchange chromatography | Solanum lycopersicum |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|
Storage Stability
| Storage Stability | Organism |
|---|---|
| -20°C, MES buffer, 4 months, without loss of activity | Solanum lycopersicum |
| 4°C, 48h, 20% residual activity | Solanum lycopersicum |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| azurine-crosslinked galactomannan + H2O | hydrolysis | Solanum lycopersicum | ? | - |
? | |
| galactoglucomannan + oligosaccharide | transglucosylation | Solanum lycopersicum | [3H]-labelled polysaccharide | - |
? | |
| Manbeta(1-4)Manbeta(1-4)Man + H2O | - |
Solanum lycopersicum | ? | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| endo-beta-D-mannanase | - |
Solanum lycopersicum |
| LeMAN4a | - |
Solanum lycopersicum |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 5 | 5.3 | - |
Solanum lycopersicum |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 3.5 | 6 | - |
Solanum lycopersicum |
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