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Literature summary for 3.2.1.78 extracted from

  • Hrmova, M.; Burton, R.A.; Biely, P.; Lahnstein, J.; Fincher, G.B.
    Hydrolysis of (1,4)-beta-D-mannans in barley (Hordeum vulgare L.) is mediated by the concerted action of (1,4)-beta-D-mannan endohydrolase and beta-D-mannosidase (2006), Biochem. J., 399, 77-90.
    View publication on PubMedView publication on EuropePMC
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General Stability

General Stability Organism
enzyme is rather unstable and steadily loses activity during the purification process. Addition of 0.2% bovine serum albumine stabilizes Hordeum vulgare

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
 additional information Km value is 0.16 mg/ml for locust-bean galactomannan Hordeum vulgare
5.3
-
 Manbeta(1-4)Manbeta(1-4)Manbeta(1-4)Manbeta(1-4)Man 30°C, pH 4.8 Hordeum vulgare

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
43000
-
 x * 43000, SDS-PAGE Hordeum vulgare

Organism

Organism UniProt Comment Textmining
Hordeum vulgare Q2I011 isoform MAN1
-

Purification (Commentary)

Purification (Comment) Organism
purification from seedling Hordeum vulgare

Source Tissue

Source Tissue Comment Organism Textmining
seedling 10 days old Hordeum vulgare
-

Storage Stability

Storage Stability Organism
-20°C, a single freeze-thawing cycle results in loss of 80% of activity. Bovine serum albumine stabilizes Hordeum vulgare

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
galactomannan + H2O
-
 Hordeum vulgare galactosyl-mannosyl oligosaccharides products show a degree of polymerization of 2-6 ?
glucomannan + H2O
-
 Hordeum vulgare glucosyl-mannosyl oligosaccharides products show a degree of polymerization of 2-6 ?
Manbeta(1-4)Manbeta(1-4)Manbeta(1-4)Manbeta(1-4)Man + H2O
-
 Hordeum vulgare Manbeta(1-4)Manbeta(1-4)Man + Manbeta(1-4)Man
-
 ?
mannan + H2O
-
 Hordeum vulgare mannosyl oligosaccharides products show a degree of polymerization of 2-6 ?
additional information during hydrolysis of 1,4-beta-manno-oligosaccharides, transglycosylation reactions occur Hordeum vulgare ?
-
 ?

Subunits

Subunits Comment Organism
? x * 43000, SDS-PAGE Hordeum vulgare

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.9
-
 Manbeta(1-4)Manbeta(1-4)Manbeta(1-4)Manbeta(1-4)Man 30°C, pH 4.8 Hordeum vulgare
12.9
-
 galactomannan 30°C, pH 4.8 Hordeum vulgare

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
4.8
-
-
 Hordeum vulgare

pI Value

Organism Comment pI Value Maximum pI Value
Hordeum vulgare isoelectric focusing
-
 7.8