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Literature summary for 3.2.1.78 extracted from

  • Lisboa, C.G.; Tonini, P.P.; Tine, M.A.; Buckeridge, M.S.
    Endo-beta -mannanase from the endosperm of seeds of Sesbania virgata (Cav.) Pers. (Leguminosae): purification, characterisation and its dual role in germination and early seedling growth (2006), Braz. J. Plant Physiol., 18, 269-280.
No PubMed abstract available
Search for more literature for 3.2.1.78

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
26000
-
 x * 26000, SDS-PAGE Sesbania virgata
36000
-
 gel filtration Sesbania virgata

Organism

Organism UniProt Comment Textmining
Sesbania virgata
-
-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
glycoprotein
-
 Sesbania virgata

Source Tissue

Source Tissue Comment Organism Textmining
endosperm
-
 Sesbania virgata
-
radicle high degree of enzyme expression during germination facilitates radicle protrusion through the surrounding endosperm by weakeing it in the region close to the radicle tip Sesbania virgata
-
seed
-
 Sesbania virgata
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
galactomannan + H2O enzyme prefers galactomannans with lower degree of galactosylation e.g. from carob gum Sesbania virgata galactosyl-mannosyl oligosaccharides
-
 ?

Subunits

Subunits Comment Organism
? x * 26000, SDS-PAGE Sesbania virgata

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
50
-
-
 Sesbania virgata

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
55
-
 15 min, almost complete loss of activity Sesbania virgata

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
3.5 4.5
-
 Sesbania virgata

pH Stability

pH Stability pH Stability Maximum Comment Organism
5
-
 30°C, 24 h, 50% loss of activity Sesbania virgata
7.8
-
 30°C, 96 h, no loss of activity Sesbania virgata

pI Value

Organism Comment pI Value Maximum pI Value
Sesbania virgata isoelectric focusing
-
 4.5