Any feedback?
Literature summary for 3.2.1.78 extracted from
- Gene cloning, expression, and characterization of a novel beta-mannanase from Bacillus circulans CGMCC 1416 (2008), J. Microbiol. Biotechnol., 18, 160-166.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression in Escherichia coli | Niallia circulans |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Ag+ | 1 mM, 2% residual activity | Niallia circulans |  |
| Cu2+ | 1 mM, 12% residual activity | Niallia circulans | |
| Hg2+ | 1 mM, 8% residual activity | Niallia circulans | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Ca2+ | 1 mM, 124% of initial activity | Niallia circulans | |
| Co2+ | 1 mM, 112% of initial activity | Niallia circulans | |
| Li+ | 1 mM, 112% of initial activity | Niallia circulans | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 31000 | - |
x * 31000, SDS-PAGE | Niallia circulans |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Niallia circulans | Q49HI4 | - |
- |
| Niallia circulans CGMCC 1416 | Q49HI4 | - |
- |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 481.5 | - |
- |
Niallia circulans |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| guar gum + H2O | - |
Niallia circulans | ? | 26% of the activity with locust bean gum | ? | |
| guar gum + H2O | - |
Niallia circulans CGMCC 1416 | ? | 26% of the activity with locust bean gum | ? | |
| locust bean gum + H2O | - |
Niallia circulans | ? | hydrolysis products are mainly oligosaccharides plus one monosaccharide | ? | |
| locust bean gum + H2O | - |
Niallia circulans CGMCC 1416 | ? | hydrolysis products are mainly oligosaccharides plus one monosaccharide | ? | |
| additional information | no substrate: soluble starch, methylcellulose | Niallia circulans | ? | - |
? | |
| additional information | no substrate: soluble starch, methylcellulose | Niallia circulans CGMCC 1416 | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 31000, SDS-PAGE | Niallia circulans |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 58 | - |
- |
Niallia circulans |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 50 | - |
retains 90% of its activity after incubation for 60 min | Niallia circulans |
| 60 | - |
but no activity remains after incubation for 20 min | Niallia circulans |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.6 | - |
- |
Niallia circulans |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 6.8 | 8 | more than 75% of maximum activity | Niallia circulans |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
