Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 3.2.1.78 extracted from

  • Do, B.C.; Dang, T.T.; Berrin, J.G.; Haltrich, D.; To, K.A.; Sigoillot, J.C.; Yamabhai, M.
    Cloning, expression in Pichia pastoris, and characterization of a thermostable GH5 mannan endo-1,4-beta-mannosidase from Aspergillus niger BK01 (2009), Microb. Cell Fact., 8, 59.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.2.1.78

Activating Compound

Activating Compound Comment Organism Structure
EDTA 236% of initial activity Aspergillus niger

Cloned(Commentary)

Cloned (Comment) Organism
expression in Pichia pastoris Aspergillus niger

Inhibitors

Inhibitors Comment Organism Structure
Mn2+ 35% of initial activity Aspergillus niger
Sodium dodecyl sulfate 20% of initial activity Aspergillus niger

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
 additional information Km values for konjac glucomannan of low viscosity, locust bean gum galactomannan, carob galactomannan of low viscosity, and 1,4-beta-D-mannan from carob are 0.6 mg/ml, 2.0 mg/ml, 2.2 mg/ml and 1.5 mg/ml, respectively Aspergillus niger

Localization

Localization Comment Organism GeneOntology No. Textmining
extracellular recombinant enzyme Aspergillus niger
-
-

Metals/Ions

Metals/Ions Comment Organism Structure
Cu2+ 110% of initial activity Aspergillus niger

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
53000
-
 x * 53000, SDS-PAGE Aspergillus niger

Organism

Organism UniProt Comment Textmining
Aspergillus niger B6V876 stran BK01
-
Aspergillus niger BK01 B6V876 stran BK01
-

Posttranslational Modification

Posttranslational Modification Comment Organism
glycoprotein
-
 Aspergillus niger

Purification (Commentary)

Purification (Comment) Organism
recombinant enzyme Aspergillus niger

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1,4-beta-D-mannan + H2O
-
 Aspergillus niger ?
-
 ?
1,4-beta-D-mannan + H2O
-
 Aspergillus niger BK01 ?
-
 ?
birch wood xylan + H2O 9.1% of the activity with locust bean gum Aspergillus niger ?
-
 ?
birch wood xylan + H2O 9.1% of the activity with locust bean gum Aspergillus niger BK01 ?
-
 ?
carob galactomannan + H2O
-
 Aspergillus niger ?
-
 ?
glucomannan + H2O
-
 Aspergillus niger ?
-
 ?
glucomannan + H2O
-
 Aspergillus niger BK01 ?
-
 ?
guar gum + H2O 18% of the activity with locust bean gum Aspergillus niger ?
-
 ?
guar gum + H2O 18% of the activity with locust bean gum Aspergillus niger BK01 ?
-
 ?
locust bean gum galactomannan + H2O
-
 Aspergillus niger mannobiose + glucosyl-mannosyl oligosaccharides main product, with low amounts of mannotriose and higher manno-oligosaccharides ?
additional information negligible activity with starch, carboxymethyl cellulose, and alpha-cellulose Aspergillus niger ?
-
 ?
additional information negligible activity with starch, carboxymethyl cellulose, and alpha-cellulose Aspergillus niger BK01 ?
-
 ?

Subunits

Subunits Comment Organism
? x * 53000, SDS-PAGE Aspergillus niger

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
80
-
-
 Aspergillus niger

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
70
-
 half-life 56 h, pH 4.0 Aspergillus niger

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
148
-
 1,4-beta-D-Mannan 70°C Aspergillus niger
215
-
 glucomannan 70°C Aspergillus niger
292
-
 carob galactomannan 70°C Aspergillus niger
330
-
 locust bean gum galactomannan 70°C Aspergillus niger

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
4.5 5
-
 Aspergillus niger