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Literature summary for 3.2.1.78 extracted from
- Efficient E. coli expression systems for the production of recombinant beta-mannanases and other bacterial extracellular enzymes (2011), Bioeng. Bugs, 2, 45-49.
Application
| Application | Comment | Organism |
|---|---|---|
| synthesis | expression in Escherichia coli based on T7 RNA polymerase promoter and tac promoter systems. Both Escherichia coli OmpA signal peptide and native Bacillus signal peptide can be used efficiently for secretion of recombinant protein. Enzyme can be harvested from whole cell lysate, periplasmic extract or culture broth 4-20 h after induction by IPTG | Bacillus subtilis |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression in Escherichia coli | Bacillus subtilis |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Bacillus subtilis | O05512 | - |
- |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 635 | - |
pH 6.0, 60°C, substrate locust bean gum | Bacillus subtilis |
| 962 | - |
pH 6.0, 60°C, substrate glucomannan | Bacillus subtilis |
| 2223 | - |
pH 6.0, 60°C, substrate 1,4-beta-D-mannan | Bacillus subtilis |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 1,4-beta-D-mannan + H2O | - |
Bacillus subtilis | ? | - |
? |  |
| glucomannan + H2O | - |
Bacillus subtilis | ? | - |
? | |
| locust bean gum + H2O | - |
Bacillus subtilis | ? | products are various manno-oligosaccharides of M2-M6 size and mannose | ? | |
| additional information | no substrate: guar gum, copra meal | Bacillus subtilis | ? | - |
? | |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 60 | - |
- |
Bacillus subtilis |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 50 | - |
pH 6.0, stable for 30 min, incubation without substrate | Bacillus subtilis |
| 60 | - |
hlaf-life 4 min | Bacillus subtilis |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6 | - |
- |
Bacillus subtilis |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 2 | 10 | 50°C, stable for 30 min | Bacillus subtilis |
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