Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 3.2.1.78 extracted from

  • Fu, X.; Huang, X.; Liu, P.; Lin, L.; Wu, G.; Li, C.; Feng, C.; Hong, Y.
    Cloning and characterization of a novel mannanase from Paenibacillus sp. BME-14 (2010), J. Microbiol. Biotechnol., 20, 518-524.
    View publication on PubMed
Search for more literature for 3.2.1.78

Protein Variants

Protein Variants Comment Organism
additional information deletion of the CBM6 domain increases the enzyme stability while enabling it to retain 80% and 60% of its initial activity after treatment at 80°C and 90°C for 30 min Paenibacillus sp.

Inhibitors

Inhibitors Comment Organism Structure
2-mercaptoethanol 1 mM, 54% residual activity Paenibacillus sp.
Ca2+ 1 mM, 83% residual activity Paenibacillus sp.
Cu2+ 1 mM, 76% residual activity Paenibacillus sp.
Hg2+ 1 mM, 4% residual activity Paenibacillus sp.
K+ 1 mM, 62% residual activity Paenibacillus sp.
Mn2+ 1 mM, 63% residual activity Paenibacillus sp.
additional information not inhibitory: Mg2+, Co2+, EDTA Paenibacillus sp.

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
 locust bean gum KM value is 3.8 mg/ml Paenibacillus sp.

Metals/Ions

Metals/Ions Comment Organism Structure
Pb2+ slight stimulation Paenibacillus sp.
Zn2+ slight stimulation Paenibacillus sp.

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
53600
-
 x * 53600, calculated Paenibacillus sp.

Organism

Organism UniProt Comment Textmining
Paenibacillus sp. C6KL35 sequence consists of a carbohydrate binding module belonging to family 6 and a family 26 catalytic domain
-
Paenibacillus sp. BME-14 C6KL35 sequence consists of a carbohydrate binding module belonging to family 6 and a family 26 catalytic domain
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
konjac glucomannan + H2O
-
 Paenibacillus sp. ?
-
 ?
konjac glucomannan + H2O
-
 Paenibacillus sp. BME-14 ?
-
 ?
locust bean gum + H2O
-
 Paenibacillus sp. ?
-
 ?
locust bean gum + H2O
-
 Paenibacillus sp. BME-14 ?
-
 ?

Subunits

Subunits Comment Organism
? x * 53600, calculated Paenibacillus sp.

Synonyms

Synonyms Comment Organism
Man26b
-
 Paenibacillus sp.

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
60
-
-
 Paenibacillus sp.

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
35 65
-
 Paenibacillus sp.
70
-
 40% of maximum activity Paenibacillus sp.

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
60
-
 half-life 12 min Paenibacillus sp.

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
4.5
-
-
 Paenibacillus sp.

pH Range

pH Minimum pH Maximum Comment Organism
4 8.5
-
 Paenibacillus sp.